To synthesize AuNPs, the optimized conditions were monitored as described earlier.29 (link) HHT extract was dissolved in distilled water at a concentration of 10 mg/mL and passed through a 0.22 µm filter before use. The gold salt (HAuCl4•3H2O) was dissolved in distilled water and used at 100 mM (stock solution). The stock solution of gold salt was added to the HHT solution at different concentrations. The reaction mixtures were incubated in a shaking incubator (MSH-20A, DAIHAN Scientific, Republic of Korea). We optimized the conditions for the synthesis of HHT-AuNPs in terms of HHT and HAuCl4•3H2O concentrations, incubation time, and reaction pH. The synthesis was first monitored by the visual color change of the reaction mixture, followed by spectral analysis. After the complete reduction of the gold salt (1.5 mM) into the nanoparticles, the nanoparticles were collected by centrifugation at 12,000 rpm for 20 min (Smart R17 Plus, Hanil Scientific Inc., Republic of Korea). The obtained nanoparticles were washed thoroughly with distilled water to remove unconverted metal ions or other constituents. Finally, the nanoparticles were collected by air-drying and used for analytical characterization and application.
Msh 20a
Manufactured by Daihan Scientific
The MSH-20A is a laboratory mixer/shaker designed for mixing and agitating liquid samples. It features a variable speed control and can accommodate a variety of sample containers.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using msh 20a
1
Synthesis and Characterization of HHT-Capped AuNPs
2
Biosynthesis of CJ-Derived Gold Nanoparticles
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To prepare CJ-AuNPs, the optimized conditions were monitored in the previous study21. CJ ethanol extract was dissolved in distilled water and passed through a 0.22 µm filter (Advantec) before use. The filtered CJ was mixed with HAuCl4·3H2O solution at different concentrations. The reaction mixture was incubated in a shaking incubator (MSH-20A; Daihan Scientific, Gangwon-do, Korea). The optimized conditions for the CJ-AuNPs biosynthesis were explored in terms of concentrations of CJ ethanol extract and HAuCl4·3H2O, incubation time, and reaction pH value. The completion of biosynthesis was decided by visualization of changed color (from yellow to purple), following determination of optical density using UV-visible (Vis) spectrophotometer (Cary 60; Agilent Technologies, Santa Clara, CA, USA). To remove the soluble materials, the mixture was centrifuged (12,000 rpm, 20 min) using a centrifuge (Smart R17 Plus, Hanil Scientific Inc., Korea), and the precipitated particles were washed with distilled water. This process was repeated four more times to purify the particles. Finally, the CJ-AuNPs were stored at 4 °C for further cell treatment.
3
Bench-scale NTP system for wastewater treatment
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A lab-scale of glow-discharge NTP system (Groon Co., Ltd., Jeonju, Korea) was manually manufactured for the experiments and had an electrical specification of 10 mA and 2.2 W. An aeration pump (Goseong valve, YP−20A, Korea) was connected to the NTP system to supply the air at room temperature (about 25 °C) with a maximum air flowrate of 20 L/min. The air flowrate was controlled by a flow meter (Dwyer, RMA−22-SSV, Michigan City, IN, USA). Two rubber lines were made on the NTP system, and the airflow was set at 10 L/min by inserting a round sparger (diameter 3 cm) into the reactor. The wastewater was reacted in a 2.5 L bottle (2 L working volume). For homogeneous radical contact in the wastewater, the reactor was vigorously mixed by using an agitator (DAIHAN Scientific, MSH−20A, Wonju, Korea). The temperature was adjusted according to an experimental design by using a water bath (DAIHAN Scientific, LSB−055S, Wonju, Korea). Figure 1 shows a schematic of the bench-scale NTP system.
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