P005091

Human Embryonal Carcinoma cell line NTERA-2 and the murine testicular cell lines TM4 (Sertoli), GC-1 (Spermatogonia), and GC-2 (Spermatocytes) were maintained in DMEM (Gibco, Paisley, UK), supplemented with 10% fetal bovine serum (FBS; Gibco BRL, Italia), 1% L- Glutamine and 1% of penicillin – streptomycin (Gibco, Paisley, UK) [25] , [26] (link), [27] (link), [28] (link). Olaparib (AZD2281) and P005091 were provided by SelleckChem. Cycloheximide, cisplatinum, H2O2 (H1009), erastin (E7781), ferrostatin-1 (SML0583) and desferoxamine (D9533) were from Sigma-Aldrich, Inc (St. Louis, CA, USA). Z-VAD-fmk (FMK001) was from MedChemExpress.

The PC3, LNCaP and the 22Rv1 prostate cancer cell lines were obtained from the American Type Culture Collection (Rockville, MD, USA). The PC3 and the LNCaP were cultured in RPMI 1640 (Gibco, Paisley, UK), supplemented with 10% fetal bovine serum (Gibco, Paisley, UK) and 1% penicillin/streptomycin (Gibco, Paisley, UK). The 22Rv1 cell lines were cutured in RPMI 1640 (Gibco, Paisley, UK), supplemented with 20% fetal bovine serum (Gibco, Paisley, UK) and 1% penicillin/streptomycin (Gibco, Paisley, UK). Olaparib (AZD2281) and P005091 were provided by SelleckChem. The androgen 5a-dihydrotestosterone (DHT), the cycloheximide and etoposide were obtained from SIGMA-Aldrich, Inc. The caspase-3 inhibitor Z-VAD-FMK was from Merck Millipore Corporation.

Ovarian cancer cell lines, OVCAR3, OV-90, PEO1 and PEO4 were purchased from ATCC (American Type Culture Collection) and were maintained, respectively, in RPMI (OVCAR3, PEO1 and PEO4) and DMEM (OV-90) (Gibco, Paisley, UK), supplemented with 20% (OVCAR-3) or 10% (OV-90, PEO1 and PEO4) fetal bovine serum (FBS; Gibco BRL, Italia), 1% L- Glutamine and 1% of penicillin – streptomycin (Gibco, Paisley, UK). Kuramochi cells were purchased from Sekisui XenoTech, LLC and were maintained in RPMI (Gibco, Paisley, UK), supplemented with 10% FBS, 1% L-Glutamine and 1% of penicillin – streptomycin (Gibco, Paisley, UK). Olaparib (AZD2281), and P005091 were provided by SelleckChem. PARG inhibitor (PDD00017273), SB-216763, cycloheximide, MG132 and cisplatin were provided by Merk Millipore. The genetic background of the ovarian cancer cells is reported in Additional File 1: Table S1.