PBMC were isolated with Ficoll-Isopaque (Lymphoprep™; Axis-Shield, Oslo, Norway) gradient centrifugation. All cells were freshly stained with the following monoclonal antibodies and analysed promptly as previously described (9 (link)): FITC-conjugated anti-CD45RO (UCHL1; BD Biosciences); PE-conjugated anti-CD25 (M-A251; BD Biosciences); PerCP-Cy5.5-conjugated anti-CD4 (SK3; BD Biosciences); and biotinylated anti-FOXP3 (236A/E7; eBioscience, San Diego, CA) and allophycocyanin–streptavidin (BD Biosciences). The second dataset (Table I ) was obtained by LSR Fortessa (BD Biosciences), using the following settings for voltage: FSC-A (319), SSC-A (335), FL1-A (CD45RO, 582), FL2-A (CD25, 478), FL3-A (CD4, 742), and FL4-A (FOXP3, 676). FlowJo (Tree Star) was used for manual gating.
Fl1 a
Manufactured by BD
The FL1-A is a piece of laboratory equipment designed for specific functions. It collects and analyzes data related to its core purpose. No further details about its intended use or interpretation of data can be provided in a concise, unbiased, and factual manner.
Automatically generated - may contain errors
Lab products found in correlation
Streptavidin allophycocyanin, by BD (2 mentions)
Ficoll isopaque lymphoprep, by Axis-Shield (2 mentions)
Fitc conjugated anti cd45ro uchl1, by BD (2 mentions)
Pe conjugated anti cd25 m a251, by BD (2 mentions)
Percp cy5.5 conjugated anti cd4 sk3, by BD (2 mentions)
Biotinylated anti foxp3 236a e7, by Thermo Fisher Scientific (2 mentions)
Fsc a, by BD (2 mentions)
Ssc a, by BD (2 mentions)
Fl2 a, by BD (2 mentions)
Fl3 a, by BD (2 mentions)
Fl4 a, by BD (2 mentions)
Lsrfortessa, by BD (2 mentions)
2 protocols using fl1 a
1
Phenotypic Analysis of T-Cell Subsets
2
Isolation and Phenotyping of Human PBMCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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PBMCs were isolated with Ficoll–Isopaque (Lymphoprep; Axis-Shield, Oslo, Norway) gradient centrifugation. All cells were freshly stained with the following mAbs and analyzed promptly as previously described (9 (link)): FITC-conjugated anti-CD45RO (UCHL1; BD Biosciences); PE-conjugated anti-CD25 (M-A251; BD Biosciences); PerCP-Cy5.5–conjugated anti-CD4 (SK3; BD Biosciences); and biotinylated anti-FOXP3 (236A/E7; eBioscience, San Diego, CA) and allophycocyanin-streptavidin (BD Biosciences). The second data set (Table I ) was obtained by LSR Fortessa (BD Biosciences), using the following settings for voltage: FSC-A (319), SSC-A (335), FL1-A (CD45RO, 582), FL2-A (CD25, 478), FL3-A (CD4, 742), and FL4-A (FOXP3, 676). FlowJo (Tree Star) was used for manual gating.
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