Micromax software

Automated activity monitors (Digiscan animal activity monitor; Omnitech Electronics, Columbus OH) were used to assess locomotor activity. The monitors were equipped with two parallel rows of 16 sensors spaced 2.5 cm apart and were controlled by a computer using Micromax software (Omnitech Electronics). Rats that had previously undergone FST were subsequently examined in the locomotor activity assay and experiments were performed using a counterbalanced design to assign drug administration groups. For systemic administration, the drug was injected 20 min prior to the locomotor test, whereas VTA drug infusions were preformed immediately prior the test – consistent with the FST experimental design. Photobeam breaks were recorded, quantified and analyzed in 10 min bins.

Automated activity monitors (Digiscan animal activity monitor; Omnitech Electronics, Columbus OH) were used to assess locomotor activity. The monitors were equipped with two parallel rows of 16 sensors spaced 2.5 cm apart and were controlled by a computer using Micromax software (Omnitech Electronics). VTA drug infusions were performed immediately prior to the test – consistent with the FST, SPT, and EPM experimental design. Photobeam breaks were recorded, quantified and analyzed in 5 min bins.