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Dmi8 automated scanning microscope

Manufactured by Leica
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The Leica DMi8 is an automated scanning microscope designed for high-performance imaging. It features a motorized stage and automated focusing capabilities, allowing for efficient and precise sample exploration.

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Lab products found in correlation

Tcs sp5 confocal microscope, by Leica (2 mentions)

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DMI8 automated microscope DMI8 Automated DMi8 microscope

2 protocols using dmi8 automated scanning microscope

1

Automated Quantification of Angiogenesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Images were acquired using the Leica DMi8 automated scanning microscope or Leica TCS SP5 confocal microscope and processed using ImageJ software (NIH). Any contrast adjustments were made in individual channels and applied evenly across the whole image in that channel. Contrast and color balance for color images was applied evenly across the whole image. AngioTool software (NIH) was used to quantify the vascular formation metrics from single channel grayscale images of vascular stains (CD31 staining). 3D reconstructions were generated using the Leica TCS SP5 confocal microscope to generate z-stacks spanning ~200um deep into the tissues and using ImageJ to construct a 3D volume from the stacks.
Velazquez J.J., LeGraw R., Moghadam F., Tan Y., Kilbourne J., Maggiore J.C., Hislop J., Liu S., Cats D., de Sousa Lopes S.M., Plaisier C., Cahan P., Kiani S, & Ebrahimkhani M.R. (2020). Gene Regulatory Network Analysis and Engineering Directs Development and Vascularization of Multilineage Human Liver Organoids. Cell systems, 12(1), 41-55.e11.
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2

Automated Quantification of Angiogenesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Images were acquired using the Leica DMi8 automated scanning microscope or Leica TCS SP5 confocal microscope and processed using ImageJ software (NIH). Any contrast adjustments were made in individual channels and applied evenly across the whole image in that channel. Contrast and color balance for color images was applied evenly across the whole image. AngioTool software (NIH) was used to quantify the vascular formation metrics from single channel grayscale images of vascular stains (CD31 staining). 3D reconstructions were generated using the Leica TCS SP5 confocal microscope to generate z-stacks spanning ~200um deep into the tissues and using ImageJ to construct a 3D volume from the stacks.
Velazquez J.J., LeGraw R., Moghadam F., Tan Y., Kilbourne J., Maggiore J.C., Hislop J., Liu S., Cats D., de Sousa Lopes S.M., Plaisier C., Cahan P., Kiani S, & Ebrahimkhani M.R. (2020). Gene Regulatory Network Analysis and Engineering Directs Development and Vascularization of Multilineage Human Liver Organoids. Cell systems, 12(1), 41-55.e11.
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