Ab10412

Manufactured by Abcam

Ab10412 is a primary antibody designed for use in various immunoassay applications. It is a rabbit polyclonal antibody that recognizes a specific target protein. The antibody is supplied in a liquid format and is suitable for further conjugation or antibody purification procedures.

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Lab products found in correlation

Ab17958, by Abcam (1 mentions) Jbw301, by Merck Group (1 mentions) Nb200 109, by Novus Biologicals (1 mentions) Sc 53993, by Santa Cruz Biotechnology (1 mentions) Ab1790, by Abcam (1 mentions) Rabbit anti acetyl histone h3, by Merck Group (1 mentions) Rabbit anti acetyl histone h4, by Merck Group (1 mentions) Rabbit anti ctcf, by Merck Group (1 mentions) Ab3613, by Abcam (1 mentions) Ab59459, by Abcam (1 mentions)

3 protocols using ab10412

Antibody Detection in Cell Signaling

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The following antibodies were used: anti-MYCN monoclonal antibodies (OP13, Calbiochem and NB200-109, Novus Biologicals); anti-MYCN monoclonal antibody (B8.4.B) (sc-53993, Santa Cruz); anti-SMC2 rabbit polyclonal antibodies (GTX10411, GeneTex; and ab10412, Abcam); anti-β-ACTIN monoclonal antibody (AC-15) (A5441, Sigma); anti-SMC4 rabbit polyclonal antibody (ab17958, Abcam); anti-CAP-D2 rabbit polyclonal antibody (A300-601A, Bethyl Laboratories); and anti-phospho-histone H2A.X (Ser139) mouse monoclonal antibody (JBW301, Millipore).

Murakami-Tonami Y., Kishida S., Takeuchi I., Katou Y., Maris J.M., Ichikawa H., Kondo Y., Sekido Y., Shirahige K., Murakami H, & Kadomatsu K. (2014). Inactivation of SMC2 shows a synergistic lethal response in MYCN-amplified neuroblastoma cells. Cell Cycle, 13(7), 1115-1131.

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Nuclear Protein Extraction and Analysis

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The nuclei (weight, 50 µg) were incubated on ice for 15 min in a series of buffers: HE (0 mM Mg²⁺ buffer), H10Mg1, and H10Mg5. After incubation, centrifugation was performed to recover nuclei. The nuclear pellets were suspended in a final sample buffer (Laemmli, 1970 (link)) and subjected to 12.5% SDS–PAGE. Subsequently, the gels were subjected to Coomassie brilliant blue (CBB) staining and Western blotting using the following antibodies: mouse anti-Rad21 (05-908; Millipore), rabbit anti-CTCF (07-729; Millipore), rabbit anti-Smc2 (ab10412; Abcam), rabbit anti-H2B (ab1790; Abcam), rabbit anti–acetyl histone H4 (06-866; Millipore), rabbit anti–acetyl histone H3 (06-599; Millipore), and goat anti–lamin A/C (sc-6215; Santa Cruz Biotechnology).

Shimamoto Y., Tamura S., Masumoto H, & Maeshima K. (2017). Nucleosome–nucleosome interactions via histone tails and linker DNA regulate nuclear rigidity. Molecular Biology of the Cell, 28(11), 1580-1589.

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Immunofluorescence and Western Blot Analysis of Mitotic Regulators

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For immunofluorescence, antibodies against α-tubulin (Sigma, T6199), cyclin B1 (Santa Cruz, sc-70898), Bub1 (Abcam, ab900), Mad2 (Santa Cruz, sc-28261), Hec1 (Abcam, ab3613) and SMC2 (Abcam, ab10412) and human CREST autoimmune serum (Antibodies, Inc., 15-235-0001) were used. For Western blot analysis, antibodies against Rad21 (Bethyl, A300-080A), SA2 (Bethyl, A300-581A), Smc1α (Bethyl, A300-055A), Smc3 (Bethyl, A300-060A), Mau2 (Abcam, ab46906), Sgo1 (Santa Cruz, sc-54329), Plk1 (Santa Cruz, sc-53418) and Hsp90 (Abcam, ab59459) were utilized. The anti-NudCL2 antibody was generated as described previously [19 (link)]. An antibody against actin (Sigma, T1978) was used as the loading control. The secondary antibodies for immunofluorescence analyses were Alexa Fluor 488-conjugated anti-rabbit or anti-mouse IgG and Alexa Fluor 568-conjugated anti-human IgG (Invitrogen). Goat anti-mouse or anti-rabbit secondary antibodies (LI-COR) conjugated with either Alexa Fluor 680 or IRDye 800 were used for Western blot analysis.

Yang Y., Wang W., Li M., Gao Y., Zhang W., Huang Y., Zhuo W., Yan X., Liu W., Wang F., Chen D, & Zhou T. (2018). NudCL2 is an Hsp90 cochaperone to regulate sister chromatid cohesion by stabilizing cohesin subunits. Cellular and Molecular Life Sciences, 76(2), 381-395.

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