Oregon green conjugated phalloidin

The effects of primary and secondary FGF stimulation on the actin skeleton of Swiss 3T3 cells and on the intracellular localization of NFκB were studied using confocal fluorescence microscopy. The cells were fixed with 4% neutral formalin, pre-incubated in blocking permeabilizing buffer (PBS with 5% BSA and 0.1% Triton X100), and then stained with Oregon green-conjugated phalloidin and TOPRO3 (both from Invitrogen, Carlsbad, CA). To study the nuclear translocation of NFκB, fixed and permeabilized cells were first incubated with rabbit anti-NFκB antibodies (Abcam, Cambridge, MA) and then stained with Alexa 488-conjugated anti-rabbit IgG antibodies (Invitrogen) and TOPRO3. Cell images were taken using Leica SP1 confocal microscope at the MMCRI confocal microscopy facility.

The effects of FGF stimulation on the actin skeleton of naïve and IL1α-pretreated Swiss 3T3 cells were studied using confocal fluorescence microscopy. The cells were fixed with 4% neutral formalin, pre-incubated in blocking permeabilizing buffer (PBS with 5% BSA and 0.1% Triton X100), and then stained with Oregon green-conjugated phalloidin and TOPRO3 (both from Invitrogen, Carlsbad, CA). Cell images were taken using Leica SP8 confocal microscope at the MMCRI confocal microscopy facility.