Polyclonal antibodies towards S6K2 and the monoclonal antibody to the EE-tag were described previously [5 (link)]. The anti-PRMT1 (07-404), anti-PRMT3 (07-256), and anti-asymmetric dimethylarginine (ASYM24) (07-414) antibodies were purchased from Upstate Biotechnology, Inc. (Boston, MA, USA). The anti-PRMT6 antibody (IMG-506) was purchased from Imgenex (Bhubaneswar, India). The anti-phospho-rpS6 (Ser240/244) (2215), anti-phospho-p70 S6K (Thr389) (p-T412 S6K) (9205), and anti-Lamin A/C (2032) were purchased from Cell Signalling Technology (Danvers, MA, USA). The anti-β-tubulin (H-235) antibodies were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA), and the anti-β-actin (AC15) antibodies were obtained from Sigma-Aldrich (Burlington, MA, USA). The Anti-Mono/DiMethyl Arginine (MMA/DMA) antibody [7E6] (ab412) was obtained from Abcam (Boston, MA, USA). The horseradish peroxidase (HRP)-conjugated anti-mouse (W4021) and anti-rabbit HRP (W4011) antibodies were purchased from Promega Corporation (Madison, WI, USA). The monoclonal antibody to the EE-tag was a gift from Dr. Julian Downward (Oncogene Biology Laboratory, The Francis Crick Institute, London, UK). The monoclonal antibody to the Myc-tag was generated at Ivan Gout’s laboratory.
Anti lamin a c 2032
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-Lamin A/C (2032) is a primary antibody produced by Cell Signaling Technology. It is designed to detect Lamin A and Lamin C proteins, which are structural components of the cell nucleus.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin ac 15, by Merck Group (1 mentions)
Anti flag, by Merck Group (1 mentions)
Anti α tubulin, by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Dojindo Laboratories (1 mentions)
Zinc protoporphyrin znpp, by MedChemExpress (1 mentions)
Horseradish peroxidase hrp conjugated anti rabbit igg antibody, by Cell Signaling Technology (1 mentions)
N acetyl l cysteine nac, by Merck Group (1 mentions)
Cm h2dcfda, by Thermo Fisher Scientific (1 mentions)
Nigericin, by Adipogen (1 mentions)
Anti β actin antibody, by Abcam (1 mentions)
γh2ax, by Cell Signaling Technology (1 mentions)
4 protocols using anti lamin a c 2032
1
Antibody Characterization for Protein Analysis
2
Antibodies for NF-κB Analysis
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(−)-DHMEQ was synthesized in our laboratory as described previously (9 ). Mouse monoclonal anti-p65 NF-κB (sc-8008), anti-p50 NF-κB (sc-8414), anti-p52 NF-κB (sc-7386), anti-c-Rel NF-κB (sc-6955), anti-GST (sc-138), and rabbit polyclonal anti-IκBα (sc-203) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit monoclonal anti-RelB (#4922), anti-NF-κB2 p100/p52 (#3017), and polyclonal anti-Lamin A/C (#2032) antibodies were purchased from Cell Signaling (Beverly, MA, USA). Mouse monoclonal anti-FLAG and anti-α-tubulin antibodies were purchased from Sigma-Aldrich (St. Louis, MO, USA).
3
Investigating Oxidative Stress Pathways
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We purchased 1,25(OH)2VD3 from Toronto Research Chemicals (North York, Ontario, Canada), nigericin from AdipoGen Life Sciences (San Diego, CA, USA), N-acetyl-l -cysteine (NAC) from Sigma-Aldrich (St. Louis, MO, USA), zinc protoporphyrin (ZnPP) from MedChemExpress (Monmouth junction, NJ, USA), anti-IL-1β antibody (MAB601) from R&D Systems (Minneapolis, MN, USA), anti-caspase-1 (D7F10), anti-NRF2 (D1Z9C), anti–HO–1 (D60G11), anti-Lamin A/C (2032), and horseradish peroxidase (HRP)-conjugated anti-rabbit IgG antibodies from Cell Signaling Technology (Danvers, MA, USA), anti-beta actin antibody from Abcam (Cambridge, MA, USA), anti-ASC (N-15) antibody from Santa Cruz Biotechnology (Santa Cruz, CA, USA), anti-GAPDH (6C5) from Calbiochem (San Diego, CA, USA), 4′,6-diamidino-2-phenylindole (DAPI) from Dojindo Laboratories (Kumamoto, Japan), Alexa Fluor 594-conjugated anti-mouse antibodies from Thermo Fisher Scientific (Waltham, MA, USA), and CM-H2DCFDA from Invitrogen (Carlsbad, CA, USA). All other chemicals were of the highest available commercial grade.
4
Western Blotting Antibody Validation
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Antibodies used for western blotting were: ARID1a/BAF250 A301-040A (Bethyl labs, 1:1000), ARID1a/BAF250 A301-041A (Bethyl labs, 1:5000), GR E20 (Santa Cruz, sc-1003, 1:500), GR D6H2L (Cell Signaling Technology, 1:1000), γH2AX (#2577; Cell Signaling Technology), PARP1 46D11 (Cell Signaling Technology), 53BP1 4937 (Cell Signaling Technology) and SFPQ ab38148 (Abcam), Anti Beta Tubulin T4026 (Sigma) or Anti Lamin A/C 2032 (Cell Signaling Technology, 1:1000). Secondary antibodies used were, ECL anti-rabbit, NA934V (GE Healthcare, UK, 1:10,000), or ECL anti-mouse, NA931V (GE Healthcare, UK, 1:10,000).
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