Jurkat

Lenti-X-293T (Human embryonic kidney cell line) were purchased from TaKaRa Bio, Inc. (Shiga, Japan). NCI-H1975, A549 (human non-small cell lung cancer), A375, SK-MEL-2 (human malignant melanoma)), and Jurkat (Human T-lymphocytes) cells were purchased from Cell Bank Australia (Shanghai, China), respectively. KLN205 (Murine lung cancer) and B16-F10 (Murine melanoma) cells were purchased from Cobioer bio (Nanjing, China). NCI-H1975, A549 and Jurkat cells were maintained in RPMI-1640 (Cat#01-100-1ACS, Biological Industries, Kibbutz Beit-Haemek, Israel) supplemented with 10% fetal bovine serum (FBS) (Cat#04-001-1ACS, Biological Industries) and 100U/ml penicillin-streptomycin (Cat#P1400, Solarbio, Beijing, China). A375, SK-MEL-2 and Lenti-X-293T cells were maintained in DMEM (Cat#01-052-1ACS, Biological Industries) supplemented with 10% FBS and 100 U/ml penicillin/streptomycin. B16-F10 was maintained in DMEM supplemented with 10% FBS and 100 U/ml penicillin/streptomycin. KLN205 was maintained in MEM (Biological Industries, Kibbutz Beit-Haemek, Israel) supplemented with 10% FBS, 100 U/ml penicillin/streptomycin, 0.1 mM non-essential amino acids (Cat#11140050, Gibco, Grand Island, NY, USA), 1 mM Sodium Pyruvate (Cat#11360070, Gibco, Grand Island, NY, USA).

Cells were grown under standard growth conditions. The following cells were used: Jurkat (T cell leukemia), MCF-7 (breast adenocarcinoma), U266 (multiple myeloma), MEL (melanoma), JeKo-1 (mantle cell lymphoma), A549 (lung carcinoma). Human mononuclear cells (MNCs) from healthy volunteers and human fibroblasts served as a negative control. All cell lines were originated from the American Type Culture Collection (ATCC). U-266, Jurkat, JeKo-1 and MEL were cultured in RPMI-1640, A549 and MCF-7 in DMEM supplemented with 10–20% fetal bovine serum (FBS), 100 units/mL L-glutamine and 1% penicillin/streptomycin/nystatin (Biological Industries Beit Haemek, Israel) at 37 °C with 5% CO₂. Jurkat and MEL cell media were supplemented with 10% FBS, U-266 was grown in the presence of 15% FBS and JeKo-1 with 20% FBS. Fibroblasts were propagated in DMEM with 20% FBS supplemented with L-glutamine and penicillin/streptomycin/nystatin.
Patients’ samples were obtained upon consent via protocol # 0038-12 approved by the IRB of Rabin Medical Center.

HEK293T cells and T-ALL cell lines; MOLT-4, and JURKAT cells, were purchased from the American Type Culture Collection, (ATCC, Manassas, VA). HEK293T cells were cultured in DMEM supplemented with 10% fetal bovine serum (FBS, Biological Industries, Israel) and 1% penicillin/streptomycin (Beyotime Biotechnology, China). MOLT-4 and JURKAT cells were cultured in RPMI-1460 media (Biological Industries, Israel) supplemented with 10% fetal bovine serum (FBS, Biological Industries, Israel), 1% L-glutamine (Hyclone, United States), and 1% penicillin/streptomycin (Beyotime Biotechnology, China). The cells in the culture were maintained at 37°C in a humidified atmosphere with 5% CO2.