Fos choline 12 fc12

All radiolabelled ligands were purchased from PerkinElmer: [¹²⁵I]sar¹-Ile⁸-angiotensin II ([¹²⁵I]Sar¹), [³H]dihydroalprenolol ([³H]DHA), [³H]dipropylcyclopentylxanthine ([³H]DPCPX) and [¹²⁵I]2β-carbomethoxy-3β-(4-iodophenyl)tropane ([¹²⁵I]RTI-55). The detergents n-dodecyl β-d-maltopyranoside (DDM) and fos-choline-12 (FC12) were purchased from Anatrace; SDS was purchased from Sigma and digitonin was purchased from Calbiochem. Anti-penta-histidine antibody conjugated to HRP (anti-pentaHis-HRP) was purchased from Qiagen. A tetracycline-inducible HEK293 cell line, T-Rex™-293 (iHEK), was purchased from Invitrogen. A tetracycline-inducible HEK293S cell line lacking N-acetylglucosaminyltransferase I (iGnTI⁻) was kindly provided by Philip J. Reeves (Massachusetts Institute of Technology) [36] (link).

Pichia pastoris strain KM71H (aox::ARG4, arg4), expression vector pPICZ-A and Zero Blunt TOPO PCR cloning kit were purchased from Invitrogen. A plasmid containing the gene for full-length human STRA6 transcript variant 2 (NM_022369.3) in the pCMV6-XL4 cloning vector, was purchased from OriGene. The pEGFP-N1 plasmid containing eGFP was purchased from Clontech. Zeocin was from Invivogen. DyLight 594 amine-reactive dye was purchased from Pierce. N-dodecyl β-D maltoside (DDM), N-decyl β-D maltoside (DM) and Fos-Choline-12 (FC-12) were from Anatrace. Cholesteryl hemisuccinate, N, N-dimethyldodecylamine N-oxide (LDAO) and dodecyl nonaoxyethylene ether (C12E9) were from Sigma. HRV 3C protease was from Sino Biological. Polyclonal antibody raised against full-length human STRA6 (#H00064220-D01P) was from Abnova. The Biacore 3000 and instrument-specific consumables (CM5 sensor chip, ethyl(dimethylaminopropyl)-carbodiimide, N-hydroxysulphosuccinimide and ethanolamine) were obtained from GE Healthcare.