Protein quantitation kit

Manufactured by Abcam

Sourced in United States

The Protein Quantitation kit is a laboratory product designed to quantify the total protein content in a sample. It provides a reliable and accurate method for determining the concentration of proteins present in solutions or extracts. The kit includes all the necessary reagents and buffers required to perform the protein quantitation analysis.

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Lab products found in correlation

Anti actin, by Merck Group (1 mentions) Halt protease inhibitor cocktail, by Thermo Fisher Scientific (1 mentions) Anti p300, by Cell Signaling Technology (1 mentions) Anti src 1, by Cell Signaling Technology (1 mentions) Anti glucocorticoid receptor, by Cell Signaling Technology (1 mentions) Anti cxcr4, by Abcam (1 mentions) Laemmli sample buffer, by Abcam (1 mentions) Enhanced chemiluminescence western blotting detection system, by GE Healthcare (1 mentions) Nitrocellulose membrane, by GE Healthcare (1 mentions) Quantity one 4, by Bio-Rad (1 mentions)

3 protocols using protein quantitation kit

Cell Lysis and Western Blot Analysis

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Cells were lysed in RIPA buffer (25mM Tris-HCl pH 7.6, 150mM NaCl,
1% sodium deoxycholate, 0.1% SDS) with Halt TM Protease
Inhibitor Cocktail (Thermo Fisher, Florence, KY, USA) on ice for 30 mis. Samples
were centrifuged at 4 °C, 13,000 g for 15 min. Collected supernatants
and protein concentrations of the supernatants were quantified using the Protein
Quantitation Kit (Abcam, Cambridge, MA, USA). Equal levels of total protein from
different samples were subjected to SDS-PAGE analysis and signals were detected
using corresponding primary and secondary antibodies. The following antibodies
(1:1000 dilution) were used: anti-CXCR4 (Abcam, #ab181020) anti-SRC-1
(Cell Signaling Technology, 128E7, #2191); anti-Glucocorticoid Receptor
(Cell Signaling Technology, D8H2, #3660); anti-p300 (Cell Signaling
Technology, C-20, sc-585); anti-actin (Sigma, A3853).

Guo B., Huang X., Cooper S, & Broxmeyer H.E. (2017). Glucocorticoid hormone-induced chromatin remodeling enhances human hematopoietic stem cell homing and engraftment. Nature medicine, 23(4), 424-428.

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Western Blotting of c-Cbl in Glioma

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Whole cell lysates were prepared from glioma tissue, and western blotting was performed as previously described (15 (link)). Protein concentration was determined with the Protein Quantitation kit (Bradford assay; Abcam), using bovine serum albumin as a standard. Lysates (20 mg) were solubilized in Laemmli sample buffer (Abcam) by boiling, and then resolved by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis, followed by electrotransfer (45V for 15 h) onto a nitrocellulose membrane (Amersham; GE Healthcare Life Sciences, Chalfont, UK), which was then incubated with the rabbit anti-c-Cbl antibody at 4°C overnight, followed by incubation with the peroxidase-conjugated anti-rabbit IgG at room temperature for 1 h. Rabbit anti-mouse monoclonal α-tubulin (cat. no. ab52866; dilution, 1:2,000; Abcam) antibody served as a loading control. The immune complexes were visualized with an enhanced chemiluminescence western blotting detection system (Amersham; GE Healthcare Life Sciences). Quantity One 4.6 software (Bio-Rad Laboratories, Inc., Hercules, CA, USA) was used for desitometry analysis.

JING Z., LI L., WANG X., WANG M., CAI Y., JIN Z, & ZHANG Y. (2016). High c-Cbl expression in gliomas is associated with tumor progression and poor prognosis. Oncology Letters, 11(4), 2787-2791.

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Extraction and Quantification of Extracellular Polymeric Substances from E. coli HB101

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Overnight cultured E. coli HB101 was washed twice with PBS and adjusted to OD₆₀₀ = 1.0. The cell suspension was treated by QACs for 4 h. Then, 15 mL of cell suspension was centrifuged and the supernatant was discarded. 5 mL of 0.05 % NaCl that has been preheated to 70 °C was added, vortexed for 1 min and centrifuged at 4500 g for 5 min. Supernatant was collected as loose EPS. 5 mL of 0.05 % NaCl was added again, and put into a 60 °C water bath for 30 min, centrifuged at 4500 g for 5 min and the supernatant collected as tight EPS (Liao et al., 2019 (link)). The content of polysaccharide was determined by the phenol‑sulfuric acid method, see details in Text S6. The protein content of EPS was determined by the Bradford method according to the instructions of the Protein Quantitation kit (Abcam, USA).

Liu C., Goh S.G., You L., Yuan Q., Mohapatra S., Gin K.Y, & Chen B. (2023). Low concentration quaternary ammonium compounds promoted antibiotic resistance gene transfer via plasmid conjugation. The Science of the Total Environment, 887, 163781.

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