Zorbax ods

Manufactured by Agilent Technologies

Sourced in United States

Zorbax ODS is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of organic compounds. It features a silica-based stationary phase with octadecylsilane (ODS) bonding, providing a reversed-phase chromatographic mode.

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Lab products found in correlation

Flexar hplc system, by PerkinElmer (1 mentions) C18 guard column, by Phenomenex (1 mentions) Quattro premier xe, by Waters Corporation (1 mentions) Varian prostar, by Agilent Technologies (1 mentions) Alliance 2695, by Waters Corporation (1 mentions) Lc 2030c 3d, by Shimadzu (1 mentions) Cell culture medium, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) Sodium acetate, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Sartorius (1 mentions) Anhydrous aluminum chloride alcl3, by Thermo Fisher Scientific (1 mentions) Inveon multimodality system, by Siemens (1 mentions)

Close spelling variants of this product

Zorbax ODS column (8 citations) Zorbax C-18 ODS Spherex column (5 citations) Zorbax ODS (C18, (2 citations) Zorbax original ODS column (2 citations) C18 reverse‐phase Zorbax ODS column (2 citations) ZORBAX ODS C18 column (2 citations)

6 protocols using zorbax ods

HPLC Analysis of Chromatographic Compounds

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Chromatographic analyses were performed on a Flexar HPLC system (Perkin Elmer Inc., Waltham, MA, USA) equipped with a quaternary pump, photodiode array detector, and automatic injection with a 15 µL sample loop. The chromatographic separations were performed using a 150 × 4.6 mm i.d., 5 µm particle size, C18 column (Zorbax ODS, Agilent Technologies, Wilmington, DE, USA) protected by a C18 guard column (150 µm, 140 Å, Phenomenex, Torrance, CA, USA) in gradient elution mode with a mobile phase obtained mixing aqueous phosphoric acid 1% w/v adjusted at pH 2.6 (Eluent A) and acetonitrile (Eluent B) at a flow rate of 1.0 mL/min (Table 1). The detection wavelength was set at 400 nm and the oven temperature was maintained at 40 ± 1 °C during the whole analysis time.

Vaz G.R., Clementino A., Bidone J., Villetti M.A., Falkembach M., Batista M., Barros P., Sonvico F, & Dora C. (2020). Curcumin and Quercetin-Loaded Nanoemulsions: Physicochemical Compatibility Study and Validation of a Simultaneous Quantification Method. Nanomaterials, 10(9), 1650.

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TBBPA Quantification by LC-ESI-MS/MS

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The amount of TBBPA-MDBPE or TBBPA was detected by a high-performance liquid chromatography (HPLC; Waters; 2695) tandem mass spectrometry system (MS/MS; Quattro Premier XE; Waters), with electrospray ionization (ESI) as the ion source (LC-ESI-MS/MS). A

20 - μ L

sample was injected into the chromatographic column (Zorbax® ODS;

150 \times 3 mm

5 μ m

; Agilent), and the compounds were separated through gradient elution. Methanol and ultrapure water with

1 mM

ammonium acetate were used as the mobile phases at a flow rate of

0.6 mL / min

. Both TBBPA-MDBPE and TBBPA were detected in negative electrospray ionization mode, and the identification of compounds was performed by comparing the retention times and selected ion pairs with the corresponding standard compounds.

Ren X.M., Yao L., Xue Q., Shi J., Zhang Q., Wang P., Fu J., Zhang A., Qu G, & Jiang G. (2020). Binding and Activity of Tetrabromobisphenol A Mono-Ether Structural Analogs to Thyroid Hormone Transport Proteins and Receptors. Environmental Health Perspectives, 128(10), 107008.

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Carnosine Quantification in Poultry Muscle

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Analysis of carnosine in muscle tissue was performed on five male and five female randomly selected broilers. Samples of tissues were prepared according to the method described by Aristoy and Toldra (2004) , and the concentration of carnosine was defined with a high-performance liquid chromatography (HPLC) device Varian Prostar (Varian Inc., Palo Alto, CA, USA) equipped with a fluorescent detector and a Zorbax ODS (Agilent, Santa Clara, CA, USA), 4.6×250 mm column. Before injection, the sample was derivatized with OPA reagent according to the method of Intarapichet and Maikhunthod (2005) .

Kralik G., Sak-Bosnar M., Grčević M, & Kralik Z. (2018). Effect of Amino Acids on Growth Performance, Carcass Characteristics, Meat Quality, and Carnosine Concentration in Broiler Chickens. The Journal of Poultry Science, 55(4), 239-248.

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Quantification of FAD and FMN in POR

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FAD and FMN were quantified with HPLC/fluorescence detection as described previously with slight modification [34 (link)]. Briefly, FAD and FMN were released from purified POR or POR mutant by boiling for 5 min. The denatured protein debris was spun down at 13000g for 10 min. FAD and FMN in the supernatant were analysed in a Waters Alliance 2695 chromatographic system in tandem with a Waters W474 fluorescence detector. Chromatographic separation of FAD and FMN was performed on a C¹⁸, 5 µm, 4.6 × 250 mm reverse-phase column (Agilent Zorbax-ODS) eluted with a linear gradient of 10 mM (NH₄)₂HPO₄ (pH 5.5) (solvent A)/methanol (solvent B) at a flow rate of 1 ml/min. Solvent B was changed from 10% to 50% (v/v) over 10 min; and changed from 50% to 10% over 1 min; then kept at 10% for 4 min. FAD and FMN fluorescence was detected by excitation at 450 nm and emission at 520 nm and quantified using standard curves constructed with flavin solutions of known concentration. Flavin content was normalized to protein amount for WT and A287P POR.

Jin Y., Chen M., Penning T.M, & Miller W.L. (2015). Electron transfer by human wild-type and A287P mutant P450 oxidoreductase assessed by transient kinetics: functional basis of P450 oxidoreductase deficiency. The Biochemical journal, 468(1), 25-31.

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Quantification of Ginsenoside Content via HPLC

Cited 1 time

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The analysis of ginsenoside content was performed using an HPLC system equipped with a binary pump (Shimadzu, LC-2030C 3D, Shimadzu Corporation, Kyoto Japan). A reverse-phase C₁₈ column (4.6 mm × 250 mm, 5°μm; ZORBAX ODS, Agilent, USA) was used for separation. Acetonitrile and water containing 0.05% phosphoric acid (V/V) were used as the mobile phase. The linear gradient was set as follows: 0~35°min, 19% acetonitrile; 35~55°min, 19~29% acetonitrile; 55~70°min, 29% acetonitrile; and 70~100°min, 29~40% acetonitrile (acetonitrile was purchased from Tianjin Biao Shi Qi Technology Development Co. Ltd, Tianjin, China; phosphoric acid was purchased from Tianjin Kemiou Chemical Reagent Co. Ltd, Tianjin, China). The column temperature, flow rate, and wavelength were 40°C, 1.0°mL°min⁻¹, and 203 nm, respectively. This method was developed by our group based on the method provided in the Chinese Pharmacopoeia (2020), which has been verified to be reliable in analysing the ginsenoside content. Further details can be found in our previous literature [13 (link), 16 (link), 24 ].

Yu J., Xu T., Lin H., Lin Y., Zhou J, & Zhang Y. (2021). Comprehensive Quality Evaluation of American Ginseng for Different Parts and Abnormal Trait Based on the Major Ginsenoside Contents and Morphological Characteristics. BioMed Research International, 2021, 8831080.

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Radiolabeled Peptide Synthesis and Evaluation

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The NOTA was purchased from Macrocyclic, Inc; protected amino acids, other peptide synthesis reagents, and resins were obtained from Nanchang tanzhenbio Co., Ltd; anhydrous aluminum chloride (AlCl₃) and sodium acetate were purchased from Alfa Aesar (China) Chemicals Co., Ltd; and trifluoroacetic acid (TFA) was obtained from Shanghai Aladdin Biochemical Technology Co., Ltd. (China). Reagents, including phosphate-buffered saline (PBS) and cell culture medium, were obtained from Sigma-Aldrich. Fetal bovine serum (FBS) was purchased from Biological Industries (BI) company. All reagents and solvents were commercial products and used without further purification. The Siemens Cyclotron produced [¹⁸F] fluoride by bombarding a [¹⁸O] H₂O target with 12.5 MeV protons. Analytical HPLC (high-performance liquid chromatography) was performed on an Agilent 1200 system with a reversed-phase column (Agilent Zorbax ODS, 250 × 4.6 mm). Mass spectra were obtained on a Q-T of a premier UPLC system equipped with an electrospray interface (ESI) (Waters, USA). A γ-counter (CAPRA-R, Capintec, Inc., Ramsey, New Jersey, USA) was used to measure the radiation value of the biological distribution experiment. Small animal imaging was performed using a micro-PET/CT scanner (Siemens Inveon Multimodality System, Germany).

Liu Z., Wen G., Huang Y., Dong Y., Wang Z., Alhaskawi A., Zhang S., Wang G., Ye Q., Zhou H., Lu H, & Dong M. (2023). [18F]AlF-NOTA-ADH-1: A new PET molecular radiotracer for imaging of N-cadherin-positive tumors. Frontiers in Oncology, 13, 1126721.

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