Guinea pig anti insulin

Manufactured by Takara Bio

Sourced in United States, Sweden

Guinea pig anti-insulin is a laboratory reagent used in research applications. It is an antibody produced in guinea pigs that specifically binds to insulin, a hormone involved in regulating blood sugar levels. This product can be utilized in various experimental procedures, such as immunoassays, to detect and quantify insulin levels.

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Lab products found in correlation

Lsm 510 confocal microscope, by Zeiss (2 mentions)

Close spelling variants of this product

Insulin (7 citations)

2 protocols using guinea pig anti insulin

Immunohistochemical Analysis of Pancreatic Islets

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Pancreata were excised, fixed in 4% paraformaldehyde, embedded in paraffin and immunostained, as described previously19. Primary antibodies against rabbit anti‐α‐gustducin (sc395; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) and guinea pig anti‐insulin (Takara Bio Inc., Shiga, Japan) were used. DyLight 594‐conjugated anti‐guinea pig immunoglobulin G (1:100) or fluorescein isothiocyanate‐conjugated anti‐rabbit (1:100; Jackson ImmunoResearch, West Grove, PA, USA) were used as secondary antibodies. TOPRO3 (1:1000) was used to label the nuclei (Molecular Probes, Eugene, OR, USA). Immunofluorescence images were obtained using a Zeiss LSM510 confocal microscope (Carl Zeiss Co., Tokyo, Japan) in confocal mode.

Udagawa H., Hiramoto M., Kawaguchi M., Uebanso T., Ohara‐Imaizumi M., Nammo T., Nishimura W, & Yasuda K. (2020). Characterization of the taste receptor‐related G‐protein, α‐gustducin, in pancreatic β‐cells. Journal of Diabetes Investigation, 11(4), 814-822.

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Immunostaining of Pancreatic Tissues

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Pancreata were excised, fixed in 4% paraformaldehyde, embedded in paraffin, and immunostained as previously described^{46 (link),47 (link)}. Anti-rabbit NECAB1 (HPA025963, Atlas Antibodies Stockholm, Sweden) and guinea pig anti-Insulin (Takara Bio Inc.) antibodies were used. FITC-conjugated anti-rabbit (1:100) (Jackson ImmunoResearch) and DyLight 594-conjugated anti-guinea pig IgG (1:100) were used as the secondary antibodies, and TOPRO3 (1:1000) was used to label the nuclei (Molecular Probes). Immunofluorescent images were obtained using a Zeiss LSM510 confocal microscope (Carl Zeiss Co., Tokyo, Japan) in confocal mode.

Udagawa H., Funahashi N., Nishimura W., Uebanso T., Kawaguchi M., Asahi R., Nakajima S., Nammo T., Hiramoto M, & Yasuda K. (2023). Glucocorticoid receptor-NECAB1 axis can negatively regulate insulin secretion in pancreatic β-cells. Scientific Reports, 13, 17958.

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