In this study, different human HCC cell lines (Huh7, HepG2) were purchased from the Shanghai Institute of Cell Biology, Chinese Academy of Sciences (Shanghai, China). The human HCC cells were cultured in Dulbecco’s Modified Eagle Medium (Gibco, Carlsbad, CA, USA) with 10% (v/v) heat-inactivated fetal bovine serum (Gibco, Paisley, Scotland), 100U/mL streptomycin and 100 U/mL penicillin at 37 °C with 5% CO2. Primary tumor cells were isolated from tumor tissues and were cultured with the special medium for primary human liver cancer culture medium (iCell Bioscience Inc, Shanghai) with 10% fetal bovine serum.
Cells were maintained at 37 °C in a humidified incubator containing 20% O2, 5% CO2 and 75% N2 in normoxia. The hypoxic condition was achieved at 37 °C with a gas mixture containing 1% O2, 94% N2 and 5% CO2 in a humidified atmosphere.
Cells were maintained at 37 °C in a humidified incubator containing 20% O2, 5% CO2 and 75% N2 in normoxia. The hypoxic condition was achieved at 37 °C with a gas mixture containing 1% O2, 94% N2 and 5% CO2 in a humidified atmosphere.