Mrs2179 tetrasodium salt

IVM, (+)-bicuculline, and MRS2179 tetrasodium salt were obtained from Tocris (R&D Systems, Inc, Minneapolis, MN). Isradipine and alexidine hydrochloride were purchased from Cayman Chemical Company (Ann Arbor, MI). PMA, carbamoylcholine chloride (carbachol), forskolin, tyrothricin, and benzalkonium chloride were obtained from Sigma-Aldrich (St. Louis, MO). Suloctidil, tomatine, isoetharine mesylate, tacrine hydrochloride, pipamperone, dyclonine hydrochloride, and desoxycorticosterone acetate were purchased from MicroSource Discovery Systems, Inc.

Acetylcholine chloride, angiotensin II, indomethacin, N-nitro-L-arginine methyl ester (L-NAME), 2-(Methylthio) adenosine 5′-trihydrogen diphosphatetrisodium (2-MeS-ADP), MRS-2395 [2,2-Dimethyl-propionic acid 3-(2-chloro-6-methylaminopurin-9-yl)-2-(2,2-dimethyl-propionyloxymethyl)-propyl ester], sodium nitroprusside (SNP) and phenylephrine hydrochloride, were purchased from Sigma Aldrich (St. Louis, MO). Clopidogrel bisulfate (Bristol-Myers Squibb Company, New York, NY, USA) was obtained by crushing Plavix tablets (75 mg). MRS-2179 tetrasodium salt [2′-Deoxy-N6-methyladenosine 3′,5′-bisphosphate tetrasodium salt] and MRS-2211 2 disodium salt ([(2-Chloro-5-nitrophenyl)azo]-5-hydroxy-6-methyl-3-[(phosphonooxy)methyl]-4-pyridinecarboxaldehyde) were purchased from Tocris (Ellisville, MO).

MRS 2179 tetrasodium salt, MRS 2365, Gö 6976, and Gö 6983 were purchased from Tocris Bioscience (Bristol, UK). Bovine serum albumin was from Roche Diagnostics and physiological salts for the experimental buffer from Merck. Cyclopiazonic acid, EGTA, and HEPES were purchased from Sigma-Aldrich (St. Louis, MO). The plasmids containing GFP-labeled PKCs and MARCKS-GFP were provided by Prof. Tobias Meyer (Stanford University) and Prof. Naoaki Saito (Kobe University), respectively. A DAG biosensor based on the C1 domain tandem repeat in PKCγ (γC1aC1b-mCherry) was created as described earlier (29 (link)), and the genetically encoded Ca²⁺ sensor R-GECO (51 (link)) was used for measurements of the cytoplasmic Ca²⁺ concentration. mCherry targeted to the plasma membrane by a CAAX motif was used as plasma membrane marker.