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Recombinant human cd26 protein fc chimera

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Recombinant human CD26 protein (Fc-chimera) is a fusion protein consisting of the extracellular domain of human CD26 (also known as dipeptidyl peptidase-4) linked to the Fc region of human immunoglobulin G. CD26 is a multifunctional cell surface glycoprotein that plays a role in immune regulation and metabolism.

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Lab products found in correlation

Hrp conjugated antibodies, by Jackson ImmunoResearch (1 mentions) Clone 30 f11, by BioLegend (1 mentions) Facscanto 2 flow cytometer, by BD (1 mentions)

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Recombinant human CD26 protein

2 protocols using recombinant human cd26 protein fc chimera

1

MERS-CoV RBM Neutralizing Antibodies

Cited 1 time
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Seven neutralizing mAbs that target the MERS CoV RBM were used in this study: human mAbs m-336, m-337, m-338 were kindly provided by D. S. Dimitrov and T. Ying. The human mAb CDC-C2, mouse mAbs F-11 and C-12 and rhesus macaque mAb JC57–11 were kindly provided by B. S. Graham (see Lingshu et al., 2021 (link); Wang et al., 2015 (link); Ying et al., 2014 (link)). Recombinant human CD26 protein (Fc-chimera) was purchased from Abcam (ab 155730), Zotal, Israel. Polyclonal rabbit anti-M13 serum was produced in the Gershoni Laboratory in-house at Tel Aviv University. HRP-conjugated antibodies were purchased from Jackson ImmunoResearch Laboratories, Inc (West Grove, PA, USA).
Uppalapati L., Roitburd-Berman A., Weiss-Ottolenghi Y., Graham B.S., Dimitrov D.S., Ying T., Failayev H., Tsfadia Y, & Gershoni J.M. (2022). Functional reconstitution of the MERS CoV receptor binding motif. Molecular Immunology, 145, 3-16.
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2

Characterization of YS110 scFv Binding

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For flow cytometric analyses, samples were collected using a FACSCanto II flow cytometer (BD Biosciences, San Jose, CA) and analyzed with FlowJo software (Treestar, Ashland, OR). Expression of YS110 scFv was analyzed by its binding with the recombinant human CD26 protein (Fc chimera) (Abcam, Cambridge, MA), which was detected using an APC anti-human IgG F(c) antibody, goat polyclonal (Rockland immunochemicals, Limerick, PA) (Figure S1). Expression of CD8, CD26, human CD45, or mouse CD45 was analyzed using an APC anti-human CD8, clone 3B5, antibody (Invitrogen, Carlsbad, CA), an APC anti-human CD26 antibody, clone BA5b (BioLegend, San Diego, CA), a PE-Cy7 anti-human CD45 antibody, clone 2D1 (BioLegend), or an APC anti-mouse CD45 antibody, clone 30-F11 (Biolegend), respectively.
Kobayashi E., Kamihara Y., Arai M., Wada A., Kikuchi S., Hatano R., Iwao N., Susukida T., Ozawa T., Adachi Y., Kishi H., Dang N.H., Yamada T., Hayakawa Y., Morimoto C, & Sato T. (2023). Development of a Novel CD26-Targeted Chimeric Antigen Receptor T-Cell Therapy for CD26-Expressing T-Cell Malignancies. Cells, 12(16), 2059.
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