Coloscreen 3 lab pack

Manufactured by ELITechGroup

Sourced in France

The ColoScreen III Lab Pack is a diagnostic tool used for the detection of occult (hidden) blood in stool samples. The product provides a simple and reliable method for the laboratory analysis of fecal samples.

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Lab products found in correlation

3 protocols using coloscreen 3 lab pack

Colitis Induction and Assessment Protocol

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Animals were observed daily for fluid intake, weight changes, and examined for signs of colitis including weight loss, diarrhea (scored on a 0–2 scale, as follows: 0 = absence, 1 = mild, 2 = severe) and rectal bleeding, assessed with the ColoScreen III Lab Pack (Elitech, Salon-de-Provence, France) and prolapse, (scored as 0= absence, 1=presence) ^{[17 (link)]}.
Four days after the last DSS cycle administration, the animals of each group were euthanized by cervical dislocation under deep CO₂ anaesthesia and underwent laparotomy. The colons were visualized and rapidly excised. The colonic tissue samples were, immediately, fixed in 4% buffered formaldehyde for histological and immunohistochemical assays, or frozen for further molecular investigations.

Silvia S., Christel R., Caroline D., Florian R., Antonella V., Roberta S., Ilaria G., Benjamin B., Laurent D., Eugenio G., Pierre D, & Giovanni L. (2016). The novel PPARγ modulator GED-0507-34 Levo ameliorates inflammation-driven intestinal fibrosis. Inflammatory bowel diseases, 22(2), 279-292.

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Chronic Colitis and Fibrosis Induction in Mice

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Chronic colitis and fibrosis were induced in mice by oral administration of 2.5% (w/v) DSS (MW: 36,000–44,000, purchased from TdB Consultancy, Uppsala, Sweden) resuspended in autoclaved tap water and administered ad libitum for three cycles (5 days DSS followed by 7 days of tap water). Control groups received tap water only. Animals were monitored daily for fluid intake, weight changes, and examined for signs of colitis including weight loss, diarrhea (scored on a 0–2 scale, as follows: 0 = absence, 1 = mild, 2 = severe), rectal bleeding, assessed with the ColoScreen III Lab Pack (Elitech, Salon-de-Provence, France) and prolapse, (scored as 0 = absence, 1 = presence).

Di Gregorio J., Sferra R., Speca S., Vetuschi A., Dubuquoy C., Desreumaux P., Pompili S., Cristiano L., Gaudio E., Flati V, & Latella G. (2017). Role of glycogen synthase kinase-3β and PPAR-γ on epithelial-to-mesenchymal transition in DSS-induced colorectal fibrosis. PLoS ONE, 12(2), e0171093.

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Chronic Colitis and Fibrosis Induction in Mice

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The mice were randomly divided into two groups: control (H^{2 (link)}O) n=5 and DSS n=15. Chronic colitis and fibrosis were induced in mice by oral administration of 2.5% (w/v) DSS (MW: 36,000-44,000, purchased from TdB Consultancy, Uppsala, Sweden) solubilized in autoclaved tap water and administered ad libitum for three cycles (5 days DSS followed by 7 days of tap water) while control groups received only tap water. Animals were monitored daily for fluid intake, weight changes, and examined for signs of colitis such as weight loss, diarrhea (scored on a 0-2 scale, as follows: 0, absence; 1, mild; 2, severe), rectal bleeding, assessed with the ColoScreen III Lab Pack (Elitech, Salon-de-Provence, France) and rectal prolapse, (scored as 0, absence; 1, presence).

Sferra R., Pompili S., Ventura L., Dubuquoy C., Speca S., Gaudio E., Latella G, & Vetuschi A. (2018). Interaction between sphingosine kinase/sphingosine 1 phosphate and transforming growth factor-β/Smads pathways in experimental intestinal fibrosis. An in vivo immunohistochemical study. European Journal of Histochemistry : EJH, 62(3), 2956.

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