Semen sample was collected by masturbation in sterilized containers. Samples were immediately kept in an incubator and after liquefaction underwent microscopic examination. Around 200 μl of each sample was kept at −80°C until the time of DNA extraction. DNA was extracted using Invisorb Spin Virus DNA Mini Kit (Stratec, Germany).
Invisorb spin virus dna mini kit
Manufactured by Stratec
Sourced in Germany
The Invisorb Spin Virus DNA Mini Kit is a lab equipment product designed for the purification of viral DNA from various sample types. It utilizes a spin column-based method to efficiently extract and concentrate viral DNA for downstream applications.
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5 protocols using invisorb spin virus dna mini kit
1
Semen DNA Extraction for Analysis
2
Viral Nucleic Acid Extraction from CSF
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Two 200 μl of each CSF specimen were subjected to DNA and RNA extraction, respectively. Viral RNA extraction was done by Invisorb® spin virus RNA mini kit (stratec-Germany) and Viral DNA extraction using Invisorb® spin virus DNA mini kit (stratec-Germany), according to the manufacturer's instructions.
3
Viral DNA Extraction from Dilutions
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Extraction of viral DNA from 1 ml of the ddH2O serial dilutions as wells as pH, salinity and sediment concentration samples were carried out using the Invisorb® Spin Virus DNA Mini Kit (Stratec biomedical, Germany). The following modifications were made to the protocol: 400 μl of lysis buffer, 400 μl of binding buffer and 20 μl of proteinase K and carrier RNA were used per sample. Samples were eluted in 60 μl of elution buffer.
4
fRuSau02 Phage Genome Sequencing
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fRuSau02 DNA was isolated from crude phage lysate with Invisorb Spin Virus DNA Mini Kit (Stratec Biomedical, Birkenfeld, Germany). Sequencing was performed at the Institute for Molecular Medicine Finland (FIMM) Technology Centre Sequencing Unit [24 ]. For next-generation sequencing, the DNA library was constructed with Nextera sample prep kit (Illumina, San Diego, CA, USA). Paired-end sequencing was done using Illumina MiSeq PE300 sequencer (Illumina, San Diego, CA, USA) with the read length of 300 nucleotides. TheA5 (Andrew And Aaron’s Awesome Assembly)-miseq integrated pipeline for de novo assembly of microbial genomes was used to obtain the genome sequence [25 (link)]. fRuSau02 sequence was submitted to GenBank with accession number MF398190.
5
Phage DNA Extraction Protocols
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Phage DNA was isolated following the protocol of the Invisorb® Spin Virus DNA Mini Kit (Stratec, Berlin, Germany) or using the protocol described earlier [19 (link)].
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