Mouse anti v5

Manufactured by Proteintech

Mouse anti-V5 is a primary antibody that specifically recognizes the V5 epitope tag. It is commonly used for the detection and purification of recombinant proteins expressed in various systems.

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Lab products found in correlation

Mouse anti flag, by Merck Group (2 mentions) Mouse anti β actin, by Cell Signaling Technology (1 mentions) Rabbit anti lc3b, by Abcam (1 mentions) Alexa fluor 488 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Rabbit anti tuj1, by Merck Group (1 mentions) Mouse anti p62, by Abcam (1 mentions) Cy5 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Alexa fluor 568 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Rabbit anti flag, by Proteintech (1 mentions) Mouse anti ha, by Proteintech (1 mentions) Anti mouse cy5, by Thermo Fisher Scientific (1 mentions) Chicken anti map2, by Abcam (1 mentions) Mouse anti parkin, by Santa Cruz Biotechnology (1 mentions) Rabbit anti tdp 43, by Proteintech (1 mentions) Rabbit anti pink1, by Novus Biologicals (1 mentions) Mouse anti gapdh, by Proteintech (1 mentions)

Close spelling variants of this product

Anti-V5 (6 citations) Anti-TM (2 citations)

2 protocols using mouse anti v5

Antibody Characterization for Western Blot and Immunostaining

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The following primary antibodies were used for Western blotting and immunostaining in this study: mouse anti-Flag (Sigma-Aldrich, F3165), rabbit anti-Flag (Proteintech, 20543-1-AP), rabbit anti-Vps4B (Proteintech, 17673-1-AP), mouse anti-P62 (Abcam, ab56416), rabbit anti-LC3B (Abcam, ab48394), mouse anti-V5 (Proteintech, 66007-1-Ig), mouse anti–glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech, 60004-1-Ig), mouse anti–β-tubulin class III (Tuj 1; Abcam, ab78078), rabbit anti–Tuj 1 (Sigma-Aldrich, T2200), and mouse anti–β-actin (Cell Signaling Technology, 3700). The horseradish peroxidase–conjugated secondary antibodies were as follows: goat anti-mouse (Sigma-Aldrich, A4416) and goat anti-rabbit (Sigma-Aldrich, A9169). The fluorophore-conjugated secondary antibodies were as follows: goat anti-rabbit Alexa Fluor 488 (Life Technologies, A11036), goat anti-mouse Alexa Fluor 488 (Life Technologies, A11029), goat anti-mouse Alexa Fluor 568 (Life Technologies, A11031), goat anti-rabbit Cy5 (Life Technologies, A10523), and goat anti-mouse Cy5 (Life Technologies, A105234).

Wang H., Wang X., Zhang K., Wang Q., Cao X., Wang Z., Zhang S., Li A., Liu K, & Fang Y. (2019). Rapid depletion of ESCRT protein Vps4 underlies injury-induced autophagic impediment and Wallerian degeneration. Science Advances, 5(2), eaav4971.

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Antibody Validation for Western Blot, IP, and IF

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The following antibodies were used for western blotting, immunoprecipitation, and immunofluorescence assays: mouse anti-FLAG (Sigma-Aldrich, F3165); mouse anti-HA (Proteintech, 66006-1); rabbit anti-HA (CST, 3724); rabbit anti-TDP-43 (Proteintech, 10782-2-AP); rabbit anti-PINK1 (Novus Biologicals, BC100-494); mouse anti-Parkin (Santa Cruz, sc-32282); mouse anti-V5 (Proteintech, 66007-1); mouse anti-GAPDH (Proteintech, 60004-1); rabbit anti-Tubulin (MBL, PM054); mouse anti-Lamin C (DSHB, LC28.26); and chicken anti-MAP2 (Abcam, ab5392). Horseradish peroxidase-conjugated secondary antibodies: anti-mouse (Sigma-Aldrich, A4416) and anti-rabbit (Sigma-Aldrich, A9169). Fluorescent secondary antibodies: anti-mouse Cy5 (Life Technologies, A10524), anti-rabbit Alexa Fluor^® 488 (Life Technologies, A11012), and anti-chicken Alexa Fluor^® 633 (Sigma-Aldrich, A-21103).

Sun X., Duan Y., Qin C., Li J.C., Duan G., Deng X., Ni J., Cao X., Xiang K., Tian K., Chen C.H., Li A, & Fang Y. (2018). Distinct multilevel misregulations of Parkin and PINK1 revealed in cell and animal models of TDP-43 proteinopathy. Cell Death & Disease, 9(10), 953.

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