Seradigm premium grade hi fbs

A549s, HEK293T cells and derivatives were cultured in complete media consisting of DMEM (GIBCO, Thermo Fisher Scientific) supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO, Thermo Fisher Scientific), 1X MEM non-essential amino acids (GIBCO, Thermo Fisher Scientific), 1 mM sodium pyruvate (GIBCO, Thermo Fisher Scientific) and maintained at 37°C under 5% CO₂. For maintenance, 800,000 cells were seeded into 10 mL of complete media every 3-4 days in 10 cm dishes. Vero E6 cells were maintained in complete DMEM (Thermo Fisher Scientific, 11965–092) containing 10% fetal bovine serum (FBS) (Thermo Fisher Scientific, 16140–071), 1% HEPES Buffer Solution (Thermo Fisher Scientific, 15630–130), and 1% penicillin-streptomycin (Thermo Fisher Scientific, 15140–122). Calu3 cells were maintained in Eagles’s Minimal Essential Medium (ATCC) containing 10% FBS and 1% penicillin-streptomycin purchased from Thermo Fisher Scientific. All cell lines were maintained at 37°C under 5% CO₂. Cells were grown in a humidified incubator at 37°C with 5% CO₂.