Six-week-old hWtEPOR, hMtEPOR and litter mate control mWtEPOR mice were anesthetized with intraperitoneal injections of 100 mg/kg ketamine (Bioniche Teoranta, Ireland) and 10 mg/kg xylazine (Akorn, IL) following mydriasis with 0.5% tropicamide ophthalmic solution (Bausch & Lomb, NY). Mice were raised onto a platform in front of the Phoenix Micron IV Imaging System (Phoenix research Labs, Pleasanton, CA), and a coupling agent, GenTeal (Novartis, NJ), was applied to the cornea. Laser photocoagulation was performed using the Micron laser module (450 mW intensity, 100 ms duration; Phoenix Research Labs, Pleasanton, CA). Three laser spots per eye were applied approximately 2 disc diameters from the optic nerve, avoiding major vessels. Disruption of Bruch’s membrane was confirmed by the appearance of a cavitation bubble. Eyes were collected three and seven days post-laser and used for either flatmounts or RT-PCR.
Phoenix micron 4 imaging system
Manufactured by Phoenix Pharmaceuticals
Sourced in United States
The Phoenix Micron IV Imaging System is a high-resolution microscopic imaging device designed for laboratory applications. It utilizes advanced optical technologies to capture detailed visual data of microscopic samples.
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Lab products found in correlation
2 protocols using phoenix micron 4 imaging system
1
Laser-Induced Murine Choroidal Neovascularization
2
In Vivo Retinal Imaging in Mice
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The retinal fundus and retinal structure were visualized using the Phoenix Micron IV imaging system (Phoenix, Pleasanton, CA, USA). The mydriatic and anesthetized mice were placed on the bracket, and using ofloxacin ointment as a medium, the optical lens (light source wavelength 830 nm) was moved close to the cornea along the visual axis. The fundus color photographs and optical coherence tomography (OCT) images were collected synchronously in real-time.
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