Transwell biocoat control inserts

Manufactured by BD

Sourced in United States

The Transwell Biocoat control inserts are a laboratory equipment component used for cellular assays. The inserts provide a permeable membrane support for cell culture applications.

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Lab products found in correlation

Primovert, by Zeiss (4 mentions)

Spelling variants of this product

Transwell inserts (350 citations) BD Biocoat control inserts (24 citations) Control insert (10 citations) BioCoat™ inserts (8 citations)

2 protocols using transwell biocoat control inserts

Cell Migration and Invasion Assay

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Cell migration and invasion assays were performed using 8-μm pore size Transwell Biocoat control inserts (migration assay) or matrigel-coated inserts, as per manufacture's instruction (Becton Dickinson). In brief, 5×10⁴ AGS cells were seeded on a transwell plate. The chambers were incubated at 37°C, 5% CO₂. After incubating for 24 h, cells on the top surface of the transwell were scraped off. Cells were fixed for 30 min with 4% paraformaldehyde, and stained for 30 min with haematoxylin. We counted the number of cells (five high-power fields) that invaded inserts under an inverted microscope (Primo Vert, Carl Zeiss). Individual experiments were repeated thrice.

Das L., Kokate S.B., Rath S., Rout N., Singh S.P., Crowe S.E., Mukhopadhyay A.K, & Bhattacharyya A. (2016). ETS2 and Twist1 promote invasiveness of Helicobacter pylori-infected gastric cancer cells by inducing Siah2. Biochemical Journal, 473(11), 1629-1640.

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Cell Migration and Invasion Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell migration and invasion assays were performed using 8-µm pore size Transwell Biocoat control inserts (migration assay) or matrigel-coated inserts, as per manufacturer’s instruction (Becton Dickinson, MA, USA). 5 × 10⁴ AGS cells in serum free media were seeded on the upper surface of 24 well transwell plate and 10% FBS-containing media was added to the lower chamber. Cells were treated with CoCl₂ or 1% O₂ alone and/or CTK7A for 24 h or left untreated. After completion of the incubation, upper surfaces of transwell were scraped off and cells in the lower surface were fixed for 30 min with 4% paraformaldehyde, and stained for 30 min with haematoxylin. Migrated and invaded cells were counted (from five different fields) under an inverted microscope (Primo Vert, Carl Zeiss, Jena, Germany).

Rath S., Das L., Kokate S.B., Ghosh N., Dixit P., Rout N., Singh S.P., Chattopadhyay S., Ashktorab H., Smoot D.T., Swamy M.M., Kundu T.K., Crowe S.E, & Bhattacharyya A. (2016). Inhibition of histone/lysine acetyltransferase activity kills CoCl2-treated and hypoxia-exposed gastric cancer cells and reduces their invasiveness. The international journal of biochemistry & cell biology, 82, 28-40.

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