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3 protocols using anti mouse gr 1

1

Immune Cell Analysis in Peritoneal Lavage

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Peritoneal lavage cells from individual mice were prepared in PBS and stained with antibodies at 4 °C for 15 min. After washing three times, the cells were resuspended in washing buffer and analyzed. Fluorescence data for 105 events from each sample were acquired on a FACS LSR II (BD Bioscience, USA) and analyzed using FlowJo software (Tomy Digital Biology Co., Ltd., Japan). The antibodies used in these experiments (neutrophils: anti-mouse-CD11b and anti-mouse-GR-1; macrophages: anti-mouse-F4/80) were purchased from eBioscience or Invitrogen (USA).
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2

Multicolor Flow Cytometry Antibodies

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Fluorophore-conjugated monoclonal anti-mouse CD3ε (FITC, 145-2C11), anti-mouse Gr1 (APC, 1A8), anti-mouse B220 (PerCP-Cy5.5, RA3-6B2), anti-mouse CD11b (PE, M1/70), anti-mouse CD45 (APC-Cy7, 30-F11), anti-mouse TCRβ (FITC, H57-597), anti-mouse TCRγδ (APC, GL3), anti-mouse CD4 (PerCP-Cy5.5, RM4-5), anti-mouse IL17A (PE, 17B7), anti-mouse CD11b (FITC, M1/70), anti-mouse CD11c (PerCP-Cy5.5, N418), anti-mouse CD49b (APC, DX5) were purchased from eBioscience (San Diego, CA, USA), Bio Legend (San Diego, CA, USA) or TONBO Bioscience (San Diego, CA, USA).
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3

Immune Cell Phenotyping Protocol

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anti-mouse Gr-1, Ly6G, CD11b and F4/80 monoclonal antibodies were purchased from eBioscience. Data were acquired using FACS LSR II (BD Bioscience) and analyzed by FlowJo software (Treestar, CA).
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