Lc ms grade methanol meoh

Pooled human liver S9 fraction (H0610.S9) and NADPH regenerating system (K5100-5) were purchased from Sekisui Xenotech (Kansas City, KS, USA). The cofactors adenosine 3′-phosphate 5′-phosphosulfate lithium salt hydrate (PAPS), acetyl-coenzyme A (acetyl-CoA), uridine diphosphate glucuronic acid (UDPGA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The glutathione (GSH) was purchased from J&K (Shanghai, China). The NIST urine (SRM 3667) and NIST plasma (SRM 1950) samples were purchased from Ango Biotechnology Co. (Shanghai, China). LC–MS grade methanol (MeOH) and water (H₂O) were purchased from Honeywell (Muskegon, MI, USA). LC–MS grade acetonitrile (ACN) was purchased from Merck (Darmstadt, Germany). Ammonium hydroxide (NH₄OH) and ammonium acetate (NH₄OAc) were purchased from Sigma (St. Louis, MO, USA). Other chemical standards were purchased from Sigma-Aldrich (St. Louis, MO), J&K (Shanghai, China), and TopScience (Shanghai, China).

LC-MS grade methanol (MeOH), LC-MS grade acetonitrile, and HPLC grade ethyl acetate were provided by Honeywell (Charlotte, USA); formic acid, hexane, 1-butanol, sodium chloride, magnesium sulfate, and zinc sulfate heptahydrate were purchased from Sigma-Aldrich (Steinheim, Germany); chloroform was provided by VWR Chemicals BDH (Avantor, USA); analytical standards of 8-isoPGF2α and 8-isoPGF2α-d4 were purchased from Cayman Chemicals (Ann Arbor, MI, USA). Deionized water was produced in the laboratory with the ELGA Veolia Chorus system (ELGA Lab Water, UK). The stock solution of 8-isoPGF2α and 8-isoPGF2α-d4 (IS) were prepared in methanol and stored at -20°C.

16:0‐LPA, 18:0‐LPA, 16:0‐alkyl‐LPA, 18:0‐alkyl‐LPA, 18:1‐alkyl‐LPA, 16:0‐LPCs, 18:1‐LPC as well as 17:0‐LPA and LPC 17:0 as internal standards were purchased from Avanti Polar Lipids. LPAs 18:2, 18:3, 20:0, 20:4 were obtained from Echelon Biosciences, Inc. (Salt Lake City, UT, USA) and LPA 18:1 from Cayman Chemical Company (Ann Arbor, MI, USA). LCMS grade water, formic acid (HCOOH), chloroform, and ammonium formate were purchased from VWR International or Merck (both Darmstadt, Germany) and LCMS grade methanol (MeOH) was purchased from Honeywell (Seelze, Germany).

Mycotoxin standards—DON, ZEN, T-2, and HT-2 toxins—were obtained from Romer Lab (Cheshire, UK). LC-MS grade methanol (MeOH) and acetonitrile (MeCN) were purchased from Honeywell (London, UK). Ultrapure water (resistivity of 18.2 MΩ-cm) was obtained from a Milli-Q system (Millipore, Burlington, MA, USA). LC-MS grade formic acid and syringe filters (PTFE, 0.22 μm pore size) were supplied by Sigma-Aldrich (St. Louis, MO, USA) and Waters (Wilmslow, UK), respectively.