Dimethylsulfoxide dmso

Tryptanthrin, amphotericin B, annexin V-FITC kit, rhodamine 123, tetrazolium salt (MTT), monodansylcadaverine, and Dulbecco’s Modified Eagle Medium (DMEM) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Dimethylsulfoxide (DMSO) was obtained from Dinâmica (Indaiatuba, SP, Brazil). Fetal bovine serum (FBS) was obtained from LGC Biotecnologia (Cotia, SP, Brazil).

The materials consisted of culture media, bacterial and fungal strains, commercial antibiotics as well as analytical grade reagents. Potato dextrose agar (PDA) and Mueller–Hinton broth were purchased from Kasvi (Kasvi, São José dos Pinhais, Brazil). Sabouraud broth and yeast extract were supplied by Himedia (Himedia, Thane, India). Microorganisms’ strains were acquired from Cefar (Cefar Diagnóstica, Jardim Taquaral, Brazil). Levofloxacin, terbinafine and chloramphenicol were obtained from EMS (EMS pharma, Hortolândia, Brazil). Dextrose, methanol, ethyl acetate, FeCl₃, dimethyl sulfoxide (DMSO), 2,3,5-triphenyltetrazoic chloride (TTC) and Folin–Ciocalteu solution were purchased from Dinâmica (Dinâmica, Indaiatuba, Brazil). Resazurin, 2,2-diphenyl-1-picrylhydrazyl (DPPH), quercetin, ascorbic acid, Trolox and gallic acid were obtained from Sigma-Aldrich (Sigma-Aldrich, Saint Louis, MO, USA). 2,4,6-Tripyridyl-s-triazine (TPTZ) was supplied by Merck (Merck, Darmstadt, Germany).