Alexa 568 conjugated donkey anti goat igg

Skin biopsies were fixed overnight in 10% formalin, embedded in paraffin, sectioned, and stained with hematoxylin and eosin. For immunohistochemistry, cultured cells and frozen sections of human skin were fixed in methanol. The cells and tissues were blocked with 10% donkey serum and incubated with primary antibodies at 4°C overnight. Antibodies included a goat polyclonal antibody against the S-protein tag (Abcam; 1:500), a mouse antiserum against human SLURP1 (Novus Biologicals; 1:100), a rat monoclonal antibody against BrdU (Abcam; 1:200), or an Alexa 568-labeled mouse monoclonal antibody against cytokeratin 14 (Abcam; 1:500). Secondary antibodies included an Alexa 568–conjugated donkey anti-goat IgG (Invitrogen; 1:800), an Alexa 488–conjugated donkey anti-mouse IgG (Invitrogen; 1:800), and an Alexa 488–conjugated donkey anti-rat IgG (Invitrogen; 1:200). DNA was visualized with DAPI. Microscopy was performed with an Axiovert 200M microscope.