Log In Sign up
The largest database of trusted experimental protocols

Iodacetamid

Manufactured by Merck Group
Visit Supplier
Sourced in Austria

Iodacetamid is a chemical compound used in various laboratory applications. It functions as an alkylating agent, which can be used for the modification and labeling of proteins and other biomolecules. The core function of Iodacetamid is to enable the selective labeling and analysis of target molecules in research and analytical settings.

Automatically generated - may contain errors

Lab products found in correlation

Trypsin lysc ms grade, by Promega (2 mentions) Dithiothreitol (dtt), by Merck Group (1 mentions)

2 protocols using iodacetamid

1

Filter-Assisted Protein Digest Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The proteins were used for a filter-assisted protein digest, as described previously [25 (link)]. Briefly, the isolated proteins were centrifuged at 4536×g for 30 min and the protein pellet dried. After dissolving in sample buffer, the protein concentration was determined with a Bradford assay and 20 µg of total protein was used for the digestion. After reduction with dithiothreitol and alkylation with iodacetamid (both Sigma-Aldrich, Vienna, Austria), proteins were digested with Trypsin/Lys-C (MS grade; Promega Corporation, Madison, WI, USA) and dried via vacuum centrifugation.
Del Favero G., Mayer R.M., Dellafiora L., Janker L., Niederstaetter L., Dall’Asta C., Gerner C, & Marko D. (2020). Structural Similarity with Cholesterol Reveals Crucial Insights into Mechanisms Sustaining the Immunomodulatory Activity of the Mycotoxin Alternariol. Cells, 9(4), 847.
+ Open protocol
+ Expand
2

Filter-Assisted Protein Digestion Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The proteins were used for a filter-assisted protein digest as described previously (15 (link)). In short, the precipitated proteins were centrifuged at 4536 × g for 30 min, the supernatant discarded and the protein pellet dried. After dissolving in sample buffer the protein concentration was determined and 20 μg of total protein was used for the digestion. After reduction with dithiothreitol and alkylation with iodacetamid (both Sigma-Aldrich) proteins were digested with Trypsin/Lys-C (MS grade; Promega Corporation, Madison, WI) and dried via vacuum centrifugation.
Neuditschko B., Janker L., Niederstaetter L., Brunmair J., Krivanek K., Izraely S., Sagi-Assif O., Meshel T., Keppler B.K., Del Favero G., Witz I.P, & Gerner C. (2019). The Challenge of Classifying Metastatic Cell Properties by Molecular Profiling Exemplified with Cutaneous Melanoma Cells and Their Cerebral Metastasis from Patient Derived Mouse Xenografts. Molecular & Cellular Proteomics : MCP, 19(3), 478-489.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!