High-quality reference standards were sourced to ensure the precision and accuracy of our analytical methodology. PZA as a certified reference material, INH (≥99% purity), and RIF (>97% purity) were procured from Sigma-Aldrich (St. Louis, MO, USA). In addition, the following reference standards and internal standards were obtained from Toronto Research Chemicals (North York, ON, Canada): ETH (98% purity), 25-D-RIF (95.06% purity), RIF-Q (95.19% purity), and 3-F-RIF (96.42% purity); ethambutol-D4 (ETH-IS; 98% purity), rifampicin-D3 (RIF-IS; 98.15% purity), pyrazinamide-15N,D3 (PZA-IS; 99.5% purity). Acetonitrile, water, and methanol were procured from Sigma-Aldrich (St. Louis, MO, USA), while isopropanol was sourced from Fisher Chemical (Fair Lawn, NJ, USA). All solvents met LCMS grade specifications. Formic acid (FA) (>99.0% purity) was supplied by Fisher Chemical. Ammonium formate (≥99% purity) was obtained from Honeywell (Morristown, NJ, USA). Blank urine of individual donors for method validation was received from BioIVT (Westbury, NY, USA).
Ethambutol d4
Manufactured by LGC
Sourced in Canada
Ethambutol-D4 is a deuterated analog of the antituberculosis drug ethambutol. It is a reference standard used for analytical testing and quality control purposes.
Automatically generated - may contain errors
Lab products found in correlation
Rifampicin d3, by LGC (3 mentions)
Methanol, by Merck Group (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
Isoniazid d4, by LGC (2 mentions)
Ethambutol, by LGC (2 mentions)
Formic acid, by Merck Group (2 mentions)
Pyrazinamide, by LGC (2 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Isopropanol, by Thermo Fisher Scientific (1 mentions)
Ammonium formate, by Honeywell (1 mentions)
Milli q deionization system, by Merck Group (1 mentions)
Hplc grade acetonitrile, by Merck Group (1 mentions)
Hplc grade methanol, by Merck Group (1 mentions)
Trifluoroacetic acid hplc grade, by Merck Group (1 mentions)
Prothionamide, by LGC (1 mentions)
Moxifloxacin d4, by LGC (1 mentions)
Trichloroacetic acid tca, by Merck Group (1 mentions)
Millipore milli q water purification system, by Merck Group (1 mentions)
Levofloxacin, by LGC (1 mentions)
Methanol, by Honeywell (1 mentions)
4 protocols using ethambutol d4
1
Precise LC-MS Quantification of Anti-TB Drugs
2
Quantitation of Anti-tuberculosis Drugs
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Pyrazinamide (lot: 100178–201104), isoniazid (lot: 100578–201502), rifampicin (lot: 130496–201403), and ethambutol dihydrochloride (lot: 100165–201705) were purchased from National Institutes for Food and Drug Control. Internal standards (ISs), pyrazinamide-D3 (lot: CRC-OW3-056), isoniazid-D4 (lot: 2-JLO-114-1), rifampicin-D3 (lot: 5-YEN-103-103-1), rifampicin-D8 (lot: NJ-07222019), and ethambutol-D4 (lot: 1-WEW-51-4), were purchased from Toronto Research Chemicals, Canada. The purities of these analytes were all above 98.0%. Molecule structures of the four analytes and corresponding ISs are presented in Figure 1 .
Methanol (HPLC grade) and acetonitrile (HPLC grade) were purchased from Merck Drugs & Biotechnology, German. Trifluoroacetic acid (HPLC grade) was purchased from Sigma Aldrich Fluka, USA. Ammonium formate (HPLC grade) was purchased from Anaqua Chemical Supply, USA. Water was prepared by a Milli-Q deionization system (Millipore, Billerica, MA, USA). Blank human serum was obtained from the physical examination population of The First Affiliated Hospital, Zhejiang University School of Medicine.
Methanol (HPLC grade) and acetonitrile (HPLC grade) were purchased from Merck Drugs & Biotechnology, German. Trifluoroacetic acid (HPLC grade) was purchased from Sigma Aldrich Fluka, USA. Ammonium formate (HPLC grade) was purchased from Anaqua Chemical Supply, USA. Water was prepared by a Milli-Q deionization system (Millipore, Billerica, MA, USA). Blank human serum was obtained from the physical examination population of The First Affiliated Hospital, Zhejiang University School of Medicine.
3
Quantification of Antibiotics in Plasma
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The reference standards of moxifloxacin, levofloxacin, prothionamide, pyrazinamide and ethambutol, as well as their internal standards of moxifloxacin-d4, levofloxacin-d8, prothionamide-d7, pyrazinamide-15 N,d3 and ethambutol-d4, were purchased from the Toronto Research Chemicals Co. (North York, ON, Canada) (Fig. 1). Methanol, acetonitrile, trichloroacetic acid (TCA), and formic acid were HPLC or ACS grade and were obtained from Sigma Chemical Co. (St Louis, MO, USA). Ultrapure water was prepared by Millipore Milli Q water purification system (Merck KGaA, Darmstadt, Germany). Eighteen healthy volunteers who had not taken the aforementioned antibiotics during the previous 3 months were enrolled in China. Each had 10 mL of blank human plasma taken and stored at -80 °C.
4
Quantification of Anti-TB Drugs
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Acetyl isoniazid, deacetyl rifabutin, desacetyl rifampicin, ethambutol, isoniazid, pyrazinamide, rifabutin and their deuterated internal standards: Acetyl isoniazid-d4 (isotopic purity: 99.4%), deacetyl rifabutin-d7 (isotopic purity: 98.5%), desacetyl rifampicin-d3 (isotopic purity: 99.6%), ethambutol-d4 (isotopic purity: 99.1%), isoniazid-d4 (isotopic purity: 99.3%), pyrazinamide-15 N,d3 (isotopic purity: 99.5%), and rifabutin-d7 (isotopic purity: 97.2%), were purchased from Toronto Research Chemicals (North York, Canada). Rifampicin reference material was purchased from the United States Pharmacopeia (Rockville, United States) and its deuterated internal standard, rifampicin-d3 (isotopic purity: 98.9%), was purchased from Toronto Research Chemicals (North York, Canada). Ammonium acetate, ascorbic acid, and dimethyl sulfoxide were purchased from Sigma-Aldrich (Darmstadt, Germany). Formic acid and ammonium bicarbonate were purchased from Merck (Darmstadt, Germany) and Acros Organics (Fair Lawn, United States), respectively. Acetonitrile and methanol were purchased from Honeywell (Charlotte, United States). LC-MS/MS grade millipore water was produced in-house (Merck-Millipore, Germany).
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