Tan was purchased from Chengdu Must Biotechnology Co., Ltd. (purity≥98%, Chengdu, China). Bleomycin sulfate (BLM) was obtained from Macklin (Shanghai, China). Pirfenidone was purchased from Solarbio (Beijing, China). The primary antibodies against E-cadherin, N-cadherin, MMP9, and collagen I were purchased from Immunoway (Plano, TX, United States). p-PI3K, PI3K, p-Akt1, and Akt1 were from Affinity Bioscience (Changzhou, China). TGF-β1, TGFBR2, α-SMA, β-actin, and all the secondary antibodies were from the Proteintech Group (Wuhan, China).
P akt1
Manufactured by Affinity Biosciences
Sourced in China, United States
P-Akt1 is a phosphorylated form of the Akt1 protein, which is a serine/threonine-protein kinase that plays a key role in multiple cellular processes, including metabolism, cell proliferation, cell survival, and cell growth. The phosphorylation of Akt1 at specific sites is a critical step in the activation of this protein.
Automatically generated - may contain errors
Lab products found in correlation
β actin, by Proteintech (1 mentions)
Tgfbr2, by Proteintech (1 mentions)
Bleomycin sulfate, by Maclin Biochemical Technology (1 mentions)
N cadherin, by Immunoway (1 mentions)
α sma, by Proteintech (1 mentions)
Tgf β1, by Proteintech (1 mentions)
P pi3k, by Affinity Biosciences (1 mentions)
E cadherin, by Immunoway (1 mentions)
Primescript rt reagent kit, by Takara Bio (1 mentions)
Bca kit, by Solarbio (1 mentions)
Glutathione peroxidase gsh px assay kit, by Nanjing Jiancheng (1 mentions)
Trypsin, by Solarbio (1 mentions)
Radioimmunoprecipitation assay (ripa), by Solarbio (1 mentions)
Primescript rt reagent kit perfect real time, by Takara Bio (1 mentions)
Annexin 5 fitc kit, by Solarbio (1 mentions)
Gsk 3β, by Affinity Biosciences (1 mentions)
Trizol, by Solarbio (1 mentions)
Gapdh, by Affinity Biosciences (1 mentions)
2 protocols using p akt1
1
Exploring Antifibrotic Mechanisms in Pulmonary Fibrosis
2
MTT Assay and Molecular Marker Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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High-glucose Dulbecco’s modified Eagle medium (DMEM), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide (MTT), frozen dimethyl sulfoxide (DMSO) stock solution, trypsin, TRIzol, RIPA, BCA kit and Annexin V-FITC kit were purchased from Solarbio (Beijing, China). Fetal bovine serum (FBS), penicillin, streptomycin, and phosphate-buffered saline (PBS) were purchased from HyClone (Logan，UT，USA). PrimeScript™ RT reagent kit was purchased from TaKaRa (Japan). Ultrapure water was purchased from Gen Pure (Germany), and 30% H2O2 was purchased from Nanjing Reagent (Nanjing,China). Lactate dehydrogenase (LDH), superoxide dismutase (SOD), aspartate aminotransferase (AST), creatine kinase (CK), and glutathione peroxidase (GSH-PX) assay kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). A PrimeScriptRT reagent kit (Perfect Real Time) was obtained from TaKaRa Biotechnology Co. (Japan). Antibodies against Akt1, p-Akt1, GSK-3β, and GAPDH were obtained from Affinity Biosciences (Cincinnati, OH, USA). Secondary antibodies were purchased from Beyotime (Shanghai, China).
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