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18 hepe

Manufactured by Cayman Chemical
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18-HEPE is a chemical compound that can be used as a research tool in the laboratory. It is a long-chain fatty acid derivative. The core function of 18-HEPE is to serve as a standard or reference material for analytical and research purposes.

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Lab products found in correlation

N formyl methionyl phenylalanine fmlp, by Merck Group (1 mentions) Hanks balanced salt solution (hbss), by Nacalai Tesque (1 mentions) Tcs sp8 confocal microscopy, by Leica (1 mentions) Acti stain 488 phalloidin, by Cytoskeleton (1 mentions) Cayman 17 18 epete, by Cayman Chemical (1 mentions) Leukotriene b4 ltb4, by Cayman Chemical (1 mentions) Triton x 100, by Nacalai Tesque (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Primaria culture dishes, by BD (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Automacs, by Miltenyi Biotec (1 mentions) Collagenase 2, by Worthington (1 mentions)

Close spelling variants of this product

18-hydroxyeicosapentaenoic acid (18-HEPE), (2 citations)

3 protocols using 18 hepe

1

Synthesis of Specialized Pro-resolving Lipid Mediators

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EPA, 18-HEPE, RvE1, and other fatty acid metabolites were obtained from Cayman Chemical. RvE2 and RvE3 were chemically synthesized as described previously (Ogawa et al., 2009 (link); Isobe et al., 2013 (link)).
Endo J., Sano M., Isobe Y., Fukuda K., Kang J.X., Arai H, & Arita M. (2014). 18-HEPE, an n-3 fatty acid metabolite released by macrophages, prevents pressure overload–induced maladaptive cardiac remodeling. The Journal of Experimental Medicine, 211(8), 1673-1687.
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2

Neutrophil Actin Polymerization Assay

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Neutrophils were purified from bone marrow as described previously.10 Briefly, bone marrow‐derived neutrophils were harvested using 62% Percoll. For the actin polymerization assay, purified neutrophils (4 × 105 cells) were suspended in HBSS (Nacalai Tesque) containing 0.2% bovine serum albumin (Sigma Aldrich) and allowed to adhere to fibronectin‐coated coverslips (Neuvitro) for 15 minutes at 37°C in a 5% CO2 incubator. Neutrophils were treated with either 1000, 100, 10, or 1 nmol/L of commercially available Cayman (±)17,18‐EpETE, BM‐3 17(S),18(R)‐EpETE, 18‐HEPE (Cayman Chemical), RvE1 (Cayman Chemical), or 0.03% (vol/vol) ethanol (vehicle control) for 15 minutes and then stimulated with 1 μmol/L N‐formyl‐methionyl‐phenylalanine (fMLP; Sigma Aldrich) or 100 nmol/L leukotriene B4 (LTB4; Cayman Chemical) for 2 minutes at 37°C in a 5% CO2 incubator. Neutrophils were fixed in 4% paraformaldehyde (Nacalai Tesque), permeabilized using 0.5% (vol/vol) Triton X‐100 (Nacalai Tesque) in PBS, and stained with 100 nmol/L Acti‐stain 488–phalloidin (Cytoskeleton) for 30 minutes at room temperature. Finally, cell nuclei were stained by incubating neutrophils with 4ʹ,6‐diamidino‐2‐phenylindole for 30 seconds at room temperature. Images were obtained with Leica TCS SP8 confocal microscopy (Leica Microsystems).
Saika A., Nagatake T., Kishino S., Park S., Honda T., Matsumoto N., Shimojou M., Morimoto S., Tiwari P., Node E., Hirata S., Hosomi K., Kabashima K., Ogawa J, & Kunisawa J. (2019). 17(S),18(R)‐epoxyeicosatetraenoic acid generated by cytochrome P450 BM‐3 from Bacillus megaterium inhibits the development of contact hypersensitivity via G‐protein‐coupled receptor 40‐mediated neutrophil suppression. FASEB bioAdvances, 2(1), 59-71.
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3

Isolation and Co-culture of Cardiac Fibroblasts

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Hearts were perfused and digested with collagenase II (Worthington Biochemical Corp). Dissociated cells were incubated with anti-CD45-conjugated microbeads (Miltenyi Biotec), followed by selective depletion of CD45+ cells using autoMACS (Miltenyi Biotec). The remaining CD45- cells were resuspended in DMEM (Life Technologies) containing 10% fetal bovine serum and placed on Primaria culture dishes (BD). After 2 h, the culture medium was changed to remove any myocytes and endothelial cells still weakly attached to the dish. After 24 h, the culture medium was changed again, and the fibroblast cultures were grown to 85% confluence, at which point the medium was replaced with DMEM containing 0.1% FBS (serum-reduced medium). After a further 24 h, the isolated fibroblasts were cultured with co-cultured macrophages and macrophage-conditioned medium with or without 18-HEPE (Cayman Chemical).
Endo J., Sano M., Isobe Y., Fukuda K., Kang J.X., Arai H, & Arita M. (2014). 18-HEPE, an n-3 fatty acid metabolite released by macrophages, prevents pressure overload–induced maladaptive cardiac remodeling. The Journal of Experimental Medicine, 211(8), 1673-1687.
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