The HUVECs were transiently transfected with SMART pools human Cav-2 siRNA (Dharmacon, USA) using Lipofectamine® reagent (Invitrogen, USA) according to the manufacturer’s instructions (Jeon et al., 2015 (link)). Green fluorescence protein (GFP) siRNA (Bioneer, Korea) was used as a control. After 48 h of transfection, the cells were used for subsequent experiments.
Gfp sirna
Manufactured by Bioneer
The GFP siRNA is a small interfering RNA (siRNA) designed to target and silence the expression of the green fluorescent protein (GFP) gene. The core function of this product is to provide a tool for researchers to study gene expression and knockdown, allowing for the investigation of cellular processes and pathways.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine reagent, by Thermo Fisher Scientific (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
Trypsin edta solution, by Welgene (1 mentions)
Linear pei 25 kda, by Polysciences (1 mentions)
Dulbecco modified eagle medium (dmem), by Polysciences (1 mentions)
Apc cy7 anti mouse cd45, by BioLegend (1 mentions)
Percp cy5.5 anti mouse cd11b, by BioLegend (1 mentions)
Citric acid, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Welgene (1 mentions)
2 protocols using gfp sirna
1
Caveolin-2 siRNA Transfection in HUVECs
2
Cytotoxicity Evaluation of Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Citric
acid was purchased from Sigma-Aldrich.
Linear PEI (25 kDa) was purchased from Polysciences, Inc. DMEM (Dulbecco’s
modified Eagle’s medium), 0.25% trypsin–EDTA solution,
PBS (pH 7.4), FBS, and P/S were purchased from WelGENE Inc. CCK-8
(cell counting kit-8) was purchased from Dojindo Molecular Technologies,
Inc. CleanCap FLuc mRNA and CleanCap EGFP mRNA were purchased from
TriLink Biotechnologies. GFP siRNA was purchased from Bioneer. Recombinant
plasmid encoding RFP was purchased from Addgene. APC/Cy7 anti-mouse
CD45 and PerCP/Cy5.5 anti-mouse CD11b were purchased from Biolegend.
acid was purchased from Sigma-Aldrich.
Linear PEI (25 kDa) was purchased from Polysciences, Inc. DMEM (Dulbecco’s
modified Eagle’s medium), 0.25% trypsin–EDTA solution,
PBS (pH 7.4), FBS, and P/S were purchased from WelGENE Inc. CCK-8
(cell counting kit-8) was purchased from Dojindo Molecular Technologies,
Inc. CleanCap FLuc mRNA and CleanCap EGFP mRNA were purchased from
TriLink Biotechnologies. GFP siRNA was purchased from Bioneer. Recombinant
plasmid encoding RFP was purchased from Addgene. APC/Cy7 anti-mouse
CD45 and PerCP/Cy5.5 anti-mouse CD11b were purchased from Biolegend.
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