LY294002, β-glycerophosphate, ascorbic acid-2-phosphate, and dexamethasone were obtained Sigma-Aldrich (St. Louis, MO, USA). XAV939 was obtained from EMD Millipore (Darmstadt, Germany). Lithium Chloride (LiCl) was purchased from Nacalai Tesque, Inc. (Kyoto, Japan). Fetal bovine serum (FBS) was purchased from Gibco (Grand Island, NY, USA). The mammalian expression vector pcDNA4/Myc was obtained from Invitrogen (Carlsbad, CA, USA). siRNA expression vectors pSilencer 4.1-CMV neo was obtained from Ambion (Austin, TX, USA). Lipofectamine 2000, G418 and puromycin were purchased from Invitrogen (Carlsbad, CA, USA). Antibodies against RunX2, Osterix, β-catenin, phospho-Akt, and β-actin were purchased from Cell Signaling Technology, Inc. (Beverly, MD, USA). The secondary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). TRIzol regent was purchased from Invitrogen (Carlsbad, CA, USA). QuantiTect Reverse Transcription kit was purchased from Qiagen (Valencia, CA, USA). SYBR Green Master Mix was purchased from Bio-Rad Laboratories (Richmond, CA, USA). Other chemicals were purchased from Sigma-Aldrich.
Antibodies against runx2
Manufactured by Cell Signaling Technology
Sourced in United States
Antibodies against RunX2 are laboratory reagents used to detect and study the expression of the RunX2 protein, a transcription factor that plays a critical role in osteoblast differentiation and bone formation. These antibodies can be used in various experimental techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to analyze the presence and distribution of RunX2 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Puromycin, by Thermo Fisher Scientific (1 mentions)
Trizol regent, by Thermo Fisher Scientific (1 mentions)
Psilencer 4.1 cmv neo, by Thermo Fisher Scientific (1 mentions)
Xav939, by Merck Group (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Phospho akt, by Cell Signaling Technology (1 mentions)
β catenin, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Ly294002, by Merck Group (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Ascorbic acid 2 phosphate, by Merck Group (1 mentions)
β glycerophosphate, by Merck Group (1 mentions)
Quantitect reverse transcription kit, by Qiagen (1 mentions)
Sybr green master mix, by Bio-Rad (1 mentions)
Antifade mounting medium with 4 6 diamidino 2 phenylindole dapi, by Vector Laboratories (1 mentions)
Fetal bovine serum (fbs), by R&D Systems (1 mentions)
Anti β actin antibody, by Merck Group (1 mentions)
Soluble recombinant rankl, by Thermo Fisher Scientific (1 mentions)
Rela p65, by Cell Signaling Technology (1 mentions)
2 protocols using antibodies against runx2
1
Osteogenic Differentiation Pathway Regulation
2
Osteoclastogenesis Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
WA (purity > 95%) was purchased from ChromaDex (Irvine, CA), dissolved in dimethyl sulfoxide (DMSO; 20 mM stock), and stored at -80°C. WRE standardized for WA (2 mM stock) was prepared in 90% ethanol as described by us previously. 26 Reagents for cell culture were purchased from Life Technologies-Thermo Fisher Scientific (Waltham, MA). Fetal bovine serum (FBS) and recombinant mouse macrophage colony-stimulating factor (M-CSF) were purchased from R&D Systems (Minneapolis, MN). Recombinant soluble RANKL was obtained from PeproTech (Cranbury, NJ). A kit for tartrate-resistant acid phosphatase (TRAP) staining was purchased from Sigma-Aldrich (St. Louis, MO). Antifade mounting medium with 4',6-diamidino-2-phenylindole (DAPI) was from Vector Laboratories (Newark, CA). Runt-related transcription factor 2 (RUNX2) antibody used for confocal microscopy was from ProteinTech (Rosemont, IL). Antibodies against RUNX2 (for immunoblotting), SOX9, and RelA (p65) were from Cell Signaling Technology (Danvers, MA). An antibody against p105/p50 of NF-κB for immunoblotting was from Santa Cruz Biotechnology (Dallas, TX). Anti-β-Actin antibody was from Sigma-Aldrich.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!