Id screen toxoplasmosis indirect multi species kit

Two commercial test kits were used for the serological detection of T. gondii during the 2-year period. Samples from 2016 were tested with the ID Screen^® Toxoplasmosis Indirect Multi-Species kit (IDVET, Montpellier, France). Samples from 2017 were tested using pigtype^® Toxoplasma Ab (Qiagen, Hilden, Germany). Both ELISA kits are suitable for the detection of antibodies from wild boar serum and assays were performed according to the manufacturer’s instructions. Doubtful results were considered negative.

Antibodies to T. gondii were detected in heart fluid (1:2 dilution) using the commercially available ID Screen® Toxoplasmosis Indirect Multi-species kit (IDvet, Grabels, France). The ELISA was performed as per manufacturer’s instructions. The optical density (OD) was recorded at 450 nm using an ELISA microplate reader (Varioskan LUX multimode, ThermoFisher Scientific). Samples presenting an S/P % ≤ 40% were considered negative. Samples ≥ 70% were considered positive. If the S/P % was between 40 and 70%, the test result was considered doubtful and repeated. All samples were tested in duplicate. Positive and negative controls provided by the manufacturers were used in each batch. In addition, positive and negative serum samples from naturally exposed foxes were used as reference controls [18 (link)]. To check for cross-reactivity with other coccidian species, reference-positive serum samples for Neospora caninum (bovine) and Hammondia hammondi (feline) were provided by the Centre for Food-borne and Animal Parasitology (CFAP) in Saskatoon. The ELISA relative sensitivity and specificity using magnetic capture PCR as a reference test were 94% and 100%, respectively [29 (link)].