Pa5 20872

Manufactured by Thermo Fisher Scientific

PA5-20872 is a laboratory equipment product from Thermo Fisher Scientific. It is designed for general laboratory use. The core function of this product is to provide a reliable and accurate solution for laboratory applications, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

2 protocols using pa5 20872

Adipose Tissue Protein Analysis

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Proteins were extracted from cells and abdominal adipose tissue using ice-cold RIPA buffer. Total proteins (30 μg) were separated by SDS–PAGE and then transferred onto PVDF membranes. The protein bands were incubated with primary antibodies against the following proteins: PGC-1α (ab188102, 1:1,000), UCP-1 (ab234430, 1:1,000), AGT (ab213705, 1:1,000), PSAT (ab232944, 1:1,000), EDNRA (ab117521, 1:1,000), mTOR (ab134903, 1:1,000), p-mTOR (ab109268, 1:1,000, Abcam), Raptor (ab40768, 1:1,000, Abcam), SREBP (ab28481, 1:1,000, Abcam), FAS (ab82419, 1:1,000, Abcam), PRDM16 (PA5-20872, 1:1,000, Thermo), PPARγ (ab272718, 1:1,000, Abcam), C/EBPα (ab32358, 1:1,000, Abcam), and GAPDH (ab8245, 1:1,000, Abcam). The next day, the bands were incubated with anti-mouse (ab6728) or anti-rabbit (ab6721) secondary antibodies for 1 h at room temperature. The visualization of proteins was detected using an ECL kit and a gel imaging system (Tanon Science & Technology Co., Ltd., China).

Song X., Han L., Lin X., Tian M., Sun F, & Feng B. (2022). Jian Pi Tiao Gan Yin alleviates obesity phenotypes through mTORC1/SREBP1 signaling in vitro and in vivo. Annals of Translational Medicine, 10(6), 291.

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Western Blot Analysis of Adipocyte Proteins

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Cell or tissue samples with equal amount of proteins (as the loading control) were separated on 12% SDS-PAGE before being transferred onto a nitrocellulose membrane. The membrane was then blocked with Superblock blocking buffer (ThermoFisher Scientific) (2 h, at room temperature) and incubated with specific primary antibody (1:200-400 dilutions, 12 h at room temperature), before washing with Tris-buffered saline-Tween solution (TBST, 3 x 15 min) and incubation with horseradish peroxidase-conjugated secondary antibody (Sigma-Aldrich) (1: 2000 -4000; 6 h, at room temperature). After washing again with TBST (3 x 15 min), the protein bands were detected in a G: BOX Chemi XT4 imaging system (Syngene) using SuperSignal WestPico Chemiluminescent Substrate (ThermoFisher Scientific).
Antibodies against UCP1 (sc-6529), peroxisome proliferator-activated receptor gamma coactivator 1-α (PGC-1α, sc-13067), adipocyte protein-2 (aP2, sc-18661) (fatty acid binding protein 4), and actin (sc-1616) were purchased from Santa Cruz Biotechnology. Antibodies against UCP1 (PA1-24894) and PRDM16 (PA5-20872) were also obtained from ThermoFisher Scientific.

Than A., Duong P.K., Zan P., Liu J., Leow M.K, & Chen P. (2020). Lancing Drug Reservoirs into Subcutaneous Fat to Combat Obesity and Associated Metabolic Diseases. Small (Weinheim an der Bergstrasse, Germany), 16(31).

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