S-tert-butylmercapto-L-cysteine (Cys(StBu); Sigma-Aldrich, Saint Louis, MO, USA), triphosgene (TCI, purity > 98.0%), (1S)-(-)-α-pinene (TCI, purity > 97.0%), PEG-OH (Sigma-Aldrich, Saint Louis, MO, USA, Mn~5000), methanesulfonyl chloride (MsCl; TCI, purity > 99.0%), triethylamine (Et3N; TCI, purity > 99.0%), chloroform (Wako, Tokyo, Japan, purity~99.0%), isopropanol (IPA; Wako, purity~98%), hexane (Wako, purity~95%), ammonia solution (Wako, ~28 wt%), 1,2,3,4-tetrahydronaphtalene (tetralin; TCI, purity > 98.0%), DL-dithiothreitol (DTT; TCI, purity > 98.0%), pyridine (Wako, purity~99.0%), butyric anhydride (TCI, purity > 98.0%), and trifluoroacetic acid (TFA; TCI, purity~98.0%) were used as received. Super-dehydrated tetrahydrofuran (THF; Kanto Chemicals, Tokyo, Japan, purity > 99.5%) and super-dehydrated N,N-dimethylformamide (DMF; Kanto Chemicals, purity > 99.5%) were further purified using a purification column (solvent dispensing system; glass contour; HANSEN & CO., Ltd., London, UK). Water for supplying the solution to the mice was prepared through the process of distillation and deionization using Milli-Q reference (Merck, Rahway, NJ, USA). Lipopolysaccharide (LPS from Escherichia coli O111:B4; Aldrich) was used as received.
Isopropanol ipa
Manufactured by Fujifilm
Sourced in Japan
Isopropanol (IPA) is a colorless, flammable liquid chemical compound commonly used in laboratory settings. It serves as a solvent and disinfectant, with the core function of cleaning and degreasing surfaces.
Automatically generated - may contain errors
Lab products found in correlation
Chloroform, by Fujifilm (1 mentions)
Ammonia solution, by Fujifilm (1 mentions)
Hexane, by Fujifilm (1 mentions)
Dl dithiothreitol, by Fujifilm (1 mentions)
Triphosgene, by Merck Group (1 mentions)
1s α pinene, by Merck Group (1 mentions)
Peg oh, by Merck Group (1 mentions)
Milli q reference, by Merck Group (1 mentions)
Heptane, by Fujifilm (1 mentions)
Ethyl acetate, by Fujifilm (1 mentions)
Trifluoroacetic acid tfa, by Fujifilm (1 mentions)
9 aminoacridine 9 aa, by Merck Group (1 mentions)
Fisher optima grade, by Thermo Fisher Scientific (1 mentions)
Methyl tert butyl ether (mtbe), by Fujifilm (1 mentions)
Phosphatidylglycerol, by Avanti Polar Lipids (1 mentions)
Phosphatidylinositol, by Avanti Polar Lipids (1 mentions)
2 protocols using isopropanol ipa
1
Synthesis and Characterization of Cys(StBu) Conjugates
2
Comprehensive LC-MS Lipid Analysis
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Ultrapure water (Fisher Optima grade, Fisher Scientific, Pittsburgh, PA) was used for liquid chromatography (LC)-MS. HPLC-grade chloroform (CHCl 3 ), methanol (MeOH), methyl-tert-butyl ether (MTBE), butanol, heptane, ethyl acetate, acetonitrile (ACN), hexane, isopropanol (IPA), and trifluoroacetic acid (TFA) were purchased from Fujifilm Wako Pure Chemical Corp. (Osaka, Japan). Proteomics-grade hydrochloric acid (HCl) was also obtained from Fujifilm Wako Pure Chemical Corp. 9-Aminoacridine (9-AA) and 8 M molecular biologygrade lithium chloride (LiCl) were purchased from Sigma-Aldrich (St. Louis, MO, USA). 2,5-Dihydroxybenzoic acid (DHB) was obtained from LaserBio Labs (Sophia Antipolis Cedex, France). All lipid internal standards were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA) and are listed in (17:0/20:4) ]; phosphatidylglycerol [PG (17:0/20:4)]; and phosphatidylinositol [PI (21:0/22:6) ]. PC (14:0/14:0) and PG (17:0/14:1) were obtained from Avanti Polar Lipids, Inc. and used as external ionization standards (Table 1-2).
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