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2 protocols using β alanine

1

Protein Expression and Apoptosis Analysis

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Fmoc-amino acids and 2-chlorotrityl chloride resin were purchased from GL Biochem (Shanghai, China). β-Alanine, 4-chloro-7-nitro-1,2,3-benzoxadiazole (NBD-Cl), crystal violet, bovine serum albumin (BSA), DAPI, propidium iodide (PI), l-phenylalanine and other chemical reagents were bought from Solarbio Science & Technology Co., Ltd. (Beijing, China). Alkaline phosphatase (ALP) and Lyso-Tracker Red were obtained from Yeasen Biotechnology (Shanghai, China). Dulbecco's modified Eagle's medium (DMEM), RPMI Medium 1640 basic and fetal bovine serum (FBS) were obtained from Gibco (Suzhou, China). Anti-γH2AX (phosphor A139) antibody and goat anti-rabbit IgG H&L (Alexa Fluor® 488) antibody were obtained from Abcam (Shanghai, China). PARP rabbit mAb, cleaved PARP (Asp214) rabbit mAb and caspase 3 rabbit antibody were purchased from Cell Signaling Technology (Shanghai, China). Anti-acetyl histone H3 and anti-acetyl histone H4 (Lys 8) antibodies were provided by Millipore (Billerica, USA). β-Actin Mouse mAb and peroxidase-conjugated affinipure goat anti-mouse/rabbit IgG (H + L) were bought from Proteintech Group Inc. (Wuhan, China). FITC Annexin V apoptosis detection kit was purchased from BD Pharmingen (San Diego, CA, USA). Dynasore was purchased from TCI Development Co., Ltd. (Shanghai, China).
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2

Molecular Mechanisms of Metabolic Regulation

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The valine, isoleucine, leucine, β-alanine, creatine, l-carnitine, and betaine were purchased from Beijing Solarbio Science and Technology Co. Ltd. (Beijing, China), while the quercetin was obtained from Aladdin (Shanghai, China). The primary antibodies: rabbit anti-phospho-adenosine monophosphate-activated protein kinase α (p-AMPKα) (#2535S), rabbit anti-phospho-acetyl CoA carboxylase (p-ACC) (#11818S), mouse anti-glucose transporter type 4 (GLUT4) (#2213S), and rabbit anti-catalase (CAT) (#14097S) were purchased from Cell Signaling Technology (Beverly, MA, USA), while the rabbit anti-carnitine palmitoyl-transferase 1B (CPT1B) (ab134988), rabbit anti-fatty acid translocase (CD36) (ab252922), and mouse anti-glutamate-cysteine ligase catalytic subunit (GCLC) (ab55435) were purchased from Abcam (Cambridge, UK). The rabbit anti-superoxide dismutase (SOD2) (BS6734) was acquired from Bioworld (Beijing, China), and the rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was purchased from ABclonal (Boston, MA, USA). The GLUT4 (GB11244) used during immunofluorescence was provided by Servicebio (Wuhan, China). The secondary antibodies, namely horseradish peroxidase-linked goat anti-rabbit IgG (Cat#485) and horseradish peroxidase-linked goat anti-mouse IgG (Cat#330), were obtained from MBL (Woburn, MA, USA).
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