MC3T3-E1 cells were cultured on the material surface with a 10 000 cells per cm2 density for 2 days to observe the cell morphology. Afterward, the cells were removed, washed with PBS, fixed in 2.5% glutaraldehyde buffer for 2 hours, dehydrated using graded alcohol concentrations (50%, 70%, 80%, 90%, and 100%) for 15 minutes each, and treated with isoamyl acetate for 5 minutes. The samples were then dried in a critical point desiccator (CPD030; Baltec, Bondoufle, France), gold-sprayed using a vacuum deposition system (Vacuum Desk III; Denton, Evry, France), and observed for both surface and cell morphology using SEM.
Vacuum desk 3
Manufactured by Denton
Sourced in France
The Vacuum Desk III is a lab equipment product designed to provide a controlled vacuum environment. It features a vacuum chamber and is capable of maintaining a specific level of reduced air pressure. The core function of the Vacuum Desk III is to create and sustain a vacuum within the designated work area.
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Lab products found in correlation
2 protocols using vacuum desk 3
1
SEM Analysis of MC3T3-E1 Cell Morphology
2
Surface-Enhanced Raman Scattering Analysis
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SERS measurements were recorded using an Ocean Optics QE65 Pro spectrometer (Ocean Optics) with Ocean Optics RPB fibre optic probe. The Ocean Optics was equipped with a 785 nm laser with 350 mW power. Spectra were acquired over a 10-20 s acquisition period using Spectra Suite software and processed using Origin 9 and MATLAB software. MTS were pipetted onto glass slides sputtered with Cr (3 nm) and Au (150 nm) in a Denton Vacuum Desk III prior to measurements.
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