Small RNA fractions (200 ng) were used for sample preparation using a FlashTag BioTin RNA labelling kit (Affymetrix, Santa Clara, CA, USA). Fragmented biotin-labelled cRNAs were further hybridized for 16 hours to an Affymetrix GeneChip miRNA 3.0 Array containing 19,724 probe-sets designed from 153 species based on miRBase version 17. After staining and washing on an Affymetrix Fluidics Station 450, arrays were scanned on an Affymetrix G3000 Gene Array Scanner. Raw data were pre-processed using Affymetrix GCOS 1.1.1 software.
Flashtag biotin rna labelling kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The FlashTag BioTin RNA Labeling Kit is a product designed for the labeling of RNA samples with biotin. This kit allows for the efficient incorporation of biotin-labeled nucleotides into RNA transcripts, enabling downstream detection and analysis applications.
Automatically generated - may contain errors
Lab products found in correlation
G3000 genearray scanner, by Thermo Fisher Scientific (1 mentions)
Fluidics station 450, by Thermo Fisher Scientific (1 mentions)
Genechip mirna 3.0 array, by Thermo Fisher Scientific (1 mentions)
Affymetrix genechip mirna 1.0 microarrays, by Thermo Fisher Scientific (1 mentions)
2100 bioanalyzer, by Agilent Technologies (1 mentions)
2 protocols using flashtag biotin rna labelling kit
1
Affymetrix miRNA Microarray Analysis
2
Profiling Adrenal Cortex miRNA
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Total RNA was extracted from cortisol-producing adenoma tissue samples and normal adrenal cortex tissue samples. All samples were assessed for enriched miRNA using an agilent 2100 Bioanalyzer. miRNAs were then labelled using the FlashTag Biotin RNA labelling kit (Affymetrix, Santa Clara, CA, USA) following the manufacturer’s protocol and then hybridized to Affymetrix GeneChip miRNA 1.0 microarrays (Affymetrix). Each miRNA was measured in duplicate.
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