A045229 2

Manufactured by Agilent Technologies

The A045229-2 is a high-performance lab equipment from Agilent Technologies. It is designed for precise and reliable measurements in a laboratory setting. The core function of this product is to provide accurate and consistent data collection and analysis capabilities for scientific research and experimentation.

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Lab products found in correlation

A32728, by Thermo Fisher Scientific (1 mentions) A32732, by Thermo Fisher Scientific (1 mentions) Ariol slide scanner, by Leica (1 mentions) Prolong gold antifade mountant with dapi, by Thermo Fisher Scientific (1 mentions) Fjk 16, by Thermo Fisher Scientific (1 mentions) Zen blue 3, by Zeiss (1 mentions) Ab15580, by Abcam (1 mentions) Epr21769, by Abcam (1 mentions) R37606, by Thermo Fisher Scientific (1 mentions) Epr3554, by Abcam (1 mentions) Epr19514, by Abcam (1 mentions) Axioscan, by Zeiss (1 mentions) Aperio imagescope 11, by Leica (1 mentions) Ab13970, by Abcam (1 mentions)

2 protocols using a045229 2

Immunofluorescent Staining of Cytokeratin and CD3

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Slides that were stained with IF after H&E staining were first decoverslipped by incubation in Xylene. Next, antigen retrieval was performed by cooking slides at 95°C for 5 min in EDTA solution pH 9 in a pressure cooker. Subsequently, slides were washed with PBS, blocked with horse serum, and incubated 1 h with pan-Cytokeratin ab (1:1800, AE1/AE3, Novus) and CD3 ab (1:400, A045229-2, Agilent). Slides were washed and incubated with secondary goat antimouse AlexaFluor647 (1:100, A32728, Thermo Fisher Scientific) and goat antirabbit AlexaFluor555 (1:100, A32732, Thermo Fisher Scientific) for 30 min. Slides were washed and mounted in ProLong™ Gold Antifade Mountant with DAPI (P36931, Thermo Fisher Scientific). Stained slides were imaged using 40× magnification using Leica Ariol slide scanner.

Yuan E., Matusiak M., Sirinukunwattana K., Varma S., Kidziński Ł, & West R. (2021). Self-Organizing Maps for Cellular In Silico Staining and Cell Substate Classification. Frontiers in Immunology, 12, 765923.

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Immunohistochemical Analysis of Mouse and Human Tissues

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Mouse tissues were collected and fixed in 10% formalin-neutral buffer. Paraffin embedding and Hematoxylin and Eosin (H&E) staining were performed at BCM Breast Center Histopathology Core. Antibodies: GFP (1:500, ab13970, Abcam), Ki67 (1:200, ab15580, Abcam), p-P27 (1:100, 71–7700, Zymed), Jagged-1 (1:100, ab7771), 2 different clones of S100A8 (1:5000, MAB3059, R&D Systems) used for mouse tissue and MRP8 (S100A8) (1:400, EPR3554, Abcam) used for human samples, CD4 (1:200, EPR19514, Abcam), CD8 (1:200, EPR21769, Abcam), F4–80 (1:200, CI:A3–1, Invitrogen), FoxP3 (1:200, FJK-16S, eBioscience), CD3 (1:400, A045229–2, Agilent), CD45 (1:200, 103102, Biolegend), and DAPI (R37606, Invitrogen). H&E and IHC images were scanned and quantified by Aperio ImageScope 11.2.0.780 (Aperio Technologies). IF images were scanned on an Axioscan and analyzed by using Zen Blue 3.1 (Zeiss). Human patient samples were purchased from Oregon Health and Science University (SR0494).

Janghorban M., Yang Y., Zhao N., Hamor C., Nguyen T.M., Zhang X.H, & Rosen J.M. (2022). Single Cell Analysis Unveils the Role of the Tumor Immune Microenvironment and Notch Signaling in Dormant Minimal Residual Disease. Cancer research, 82(5), 885-899.

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