Both indica rice and strain fermentation solution are provided by Jinjian Rice Industry Co., Ltd. (Changde, Hunan, China). The strain fermentation solution consists of a microbial fermentation system composed of lactic acid bacteria and yeast, with lactic acid bacteria concentration of 1.0 × 108 to 1.0 × 109 CFU/mL and yeast concentration of 1.0 × 104 to 1.0 × 105 CFU/mL. Sodium chloride (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China), casein tryptone (Solebro Technology Co., Ltd., Beijing, China), yeast (Auboxing Biotechnology Co., Ltd., Beijing, China), and agar (McLean Biochemical Technology Co., Ltd., Shanghai, China) were of analytical grade.
Agar
Manufactured by Maclin Biochemical Technology
Sourced in China
Agar is a gelatinous substance derived from certain red algae. It is commonly used as a solidifying agent in microbiological culture media, providing a firm, transparent support for the growth of microorganisms.
Automatically generated - may contain errors
Lab products found in correlation
Tryptone, by Maclin Biochemical Technology (9 mentions)
Sodium chloride (nacl), by Maclin Biochemical Technology (9 mentions)
Sodium chloride (nacl), by Sinopharm (4 mentions)
Sodium periodate, by Maclin Biochemical Technology (1 mentions)
Ethylene glycol, by Maclin Biochemical Technology (1 mentions)
E coli cmcc b 44103, by China Center of Industrial Culture Collection (1 mentions)
S aureus atcc 25923, by China Center of Industrial Culture Collection (1 mentions)
Tryptic soy agar, by Beijing Land Bridge Technology (1 mentions)
Tryptone soya broth (tsb), by Beijing Land Bridge Technology (1 mentions)
Calcium chloride, by Maclin Biochemical Technology (1 mentions)
Cholesterol, by Maclin Biochemical Technology (1 mentions)
Peptone, by Maclin Biochemical Technology (1 mentions)
Tg assay kit, by Nanjing Jiancheng (1 mentions)
Sodium hydroxide (naoh), by Sinopharm (1 mentions)
Oil red o staining kit, by Leagene (1 mentions)
Cat assay kit, by Nanjing Jiancheng (1 mentions)
4 protocols using agar
1
Indica Rice Fermentation Protocol
2
Nano-Textured Sc2O3-MgO Antimicrobial Nanofibers
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Magnesium (Ⅱ) chloride hexahydrate (MgCl2·6H2O), scandium (Ⅲ) chloride hexahydrate (ScCl3·6H2O), and ammonium carbonate ((NH4)2CO3) purchased from Shanghai McLean Biochemical Technology Co., Ltd. (Shanghai, China) were used to prepare the nano-textured Sc2O3-MgO. 2-2-2-Trifluoroethanol (TFE, Analytical reagent) obtained from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China) was used as spinning solvents. Polycaprolactone (PCL, 80,000) and polyvinyl pyrrolidone (PVP, K-30, 58,000) purchased from Baoqian Plastic Chemical Material Co., Ltd. (Hangzhou, China) and Aladdin Biochemical Technology Co., Ltd. (Shanghai, China) were used to prepare nanofibers. The bacteria used in this experiment was Escherichia coli (E. coli, ATCC 25,922, Shanghai Luwei Technology Co., Ltd. (Shanghai, China)). In addition, agar, tryptone, NaCl, and yeast extract powder purchased from Shanghai Maclean Biochemical Technology Co., Ltd. (Shanghai, China) were also used for the antibacterial tests. All reagents were analytically pure and were used without further processing or purification.
3
Antibacterial Efficacy Evaluation Protocol
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Agar, gentamicin sulfate (AR), ethylene glycol (AR), sodium cyanoborohydride and sodium periodate (AR) were all purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). E. coli (CMCC(B) 44103) and S. aureus (ATCC 25923) were all obtained from China Center of Industrial Culture Collection, Beijing, China. Tryptic soy Agar (TSA) and Tryptone soya broth (TSB) were obtained from Beijing Land Bridge Technology Co., Ltd. (Beijing, China).
4
Anthocyanin and Lysine Protocol
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Anthocyanin was purchased from Tianjin Jianfeng Natural products R & D Co., Ltd. (Tianjin, China); lysine was purchased from Shanghai aladdin Biochemical Technology Co., Ltd. (Shanghai, China); calcium chloride, agar, cholesterol, and peptone were purchased from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China); NaOH and NaCl were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China); an Oil Red O staining kit was purchased from Beijing Leagene Biotechnology Co., Ltd. (Beijing, China); and TG assay kits, NEFA assay kits, MDA, and CAT assay kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). All chemicals were used without purification.
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