The cell apoptosis was determined using Annexin V-uorescein isothiocyanate (FITC)/propidium iodide (PI) double staining (BD Biosciences). After treatment for 48 h, cells were harvested and resuspended in 200 µL Annexin-binding buffer. Then, the cells were incubated with 10 µL Annexin V-FITC and 5 µL PI for 30 min in the dark. The stained cells were examined by a FACScan ow cytometer (BD Biosciences).
Annexin 5 uorescein isothiocyanate fitc propidium iodide double staining
Manufactured by BD
Annexin V-FITC/PI double staining is a laboratory technique used to detect and quantify apoptosis and necrosis in cell samples. Annexin V binds to phosphatidylserine, which is externalized during apoptosis, and FITC is used as a fluorescent label. Propidium iodide (PI) is a DNA-binding dye that stains cells with compromised cell membranes, indicating necrosis. The combination of Annexin V-FITC and PI allows for the simultaneous detection of apoptotic and necrotic cells in a single sample.
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2 protocols using annexin 5 uorescein isothiocyanate fitc propidium iodide double staining
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Apoptosis Quantification by Flow Cytometry
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Apoptosis Quantification by Annexin V-FITC/PI
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The cell apoptosis was determined using Annexin V-uorescein isothiocyanate (FITC)/propidium iodide (PI) double staining (BD Biosciences). After treatment for 48 h, cells were harvested and resuspended in 200 μL Annexin-binding buffer. Then, the cells were incubated with 10 μL Annexin V-FITC and 5 μL PI for 30 min in the dark. The stained cells were examined by a FACScan ow cytometer (BD Biosciences).
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