Starch agar medium

Manufactured by HiMedia

Sourced in India

Starch agar medium is a culture medium used for the detection and identification of microorganisms that produce the enzyme amylase, which breaks down starch. The medium contains starch as a carbon source and agar as a solidifying agent.

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Lab products found in correlation

Gram s iodine, by HiMedia (1 mentions) Skim milk agar, by HiMedia (1 mentions) Blood agar medium, by HiMedia (1 mentions) Triple sugar iron agar, by HiMedia (1 mentions) Egg yolk agar medium, by HiMedia (1 mentions) Phosphate buffer saline (pbs), by HiMedia (1 mentions) Sim medium, by HiMedia (1 mentions) Lauria bertani broth, by HiMedia (1 mentions) Coomassie brilliant blue, by HiMedia (1 mentions) Nutrient agar, by HiMedia (1 mentions) Sodium acetate, by HiMedia (1 mentions)

2 protocols using starch agar medium

Extracellular Enzyme Production in Aeromonas hydrophila

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Production of extracellular enzymes such as amylase, lipase, and protease was screened in A. hydrophila Ah17. Briefly, for amylase activity, the isolate was patched on starch agar medium (HiMedia) and incubated at 37ºC. After incubation, the surface of the culture was flooded with Gram's iodine (HiMedia), and appearance of the zone of clearance around the colonies was indicated as amylase‐positive isolate (Yang & Fang, 2003).
For lipase activity, the isolate was patched on tributyrin agar base (HiMedia) containing 10 ml of tributyrin and incubated at 37°C. The appearance of the zone of clearance around the colonies was indicated as lipase‐positive isolate (Collee, Duguid, Fraser, Marmion, & Simmons, 1996).
For proteolytic activity, the isolate was patched on skim milk agar (HiMedia) and incubated at 37ºC. The appearance of the zone of clearance around the colonies was indicated as protease‐positive isolate (Yang & Fang, 2003). A. hydrophila ATCC 7966 was used as the positive control for the study.

Samayanpaulraj V., Sivaramapillai M., Palani S.N., Govindaraj K., Velu V, & Ramesh U. (2020). Identification and characterization of virulent Aeromonas hydrophila Ah17 from infected Channa striata in river Cauvery and in vitro evaluation of shrimp chitosan. Food Science & Nutrition, 8(2), 1272-1283.

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Soil Microbial Diversity in Nepal

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This study was carried out in Central Campus of Technology Hattisar Dharan and Regional Agricultural Research Station (RARS) Tarhara from October 2018 to March 2019. Soil samples were collected from different altitudes ranging from Itahari (374 ft.), Tarhara (418 ft.), Dharan (1272 ft.) and Vedetar (5140 ft.) of Koshi Zone, Nepal. From each geographical region 25 different soil samples were collected and in total 100 different soil samples from four regions were collected. Soil samples weighing 10gm was collected in sterile plastic bags from 3-5cm depth and transported into the laboratory at 4°C. Materials used in this experiment were Sodium acetate (HiMedia, India), Lauria Bertani (LB) broth (HiMedia, India). Nutrient Agar (HiMedia, India), Coomassie brilliant blue (HiMedia, India), Phosphate buffer saline (HiMedia, India), Grams staining reagents (HiMedia, India), MR-VP medium and reagents (HiMedia, India), Indole medium (HiMedia, India), Citrate agar medium (HiMedia, India), Gelatin agar medium (HiMedia, India), Starch agar medium (HiMedia, India), Egg yolk agar medium (HiMedia, India), Blood agar medium (HiMedia, India), Carbohydrate fermentation broth (HiMedia, India), SIM medium (HiMedia, India), Triple sugar iron agar (HiMedia, India).

Limbu J., Shrestha B.K., Shakya J., Khatri S.B., & Khanal H. (2020). Isolation of Bacillus thuringiensis and its Insecticidal Effect against Galleria mellonella. Himalayan Journal of Science and Technology.

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