Rabbit polyclonal anti cysc

The rats were euthanized at 6 or 24 hours after the last session of HBO preconditioning. Immunofluorescent staining protocol is described in Methods in the online-only Data Supplement. The following antibodies were used: rabbit polyclonal anti-CysC (Epitomics, Inc), rabbit polyclonal anti-cathepsin B (Abcam), mouse monoclonal anti-NeuN (Abcam), and mouse monoclonal anti-glial fibrillary acidic protein (Sigma-Aldrich). DAPI (4',6-diamidino-2-phenylindole; Sigma-Aldrich) was used for nuclei staining. Fluorescent signals in the right cortex 2 mm from the midline (presumed penumbra region if MCAO injury was induced) were detected using confocal laser scanning microscopy (FV1000; Olympus).

Western blot was performed as previously described. 11 (link) The designated region of cerebral cortexes (from bregma -2.0 to +3.0 mm, presumably the penumbra region after middle cerebral ischemia) was harvested as previously described. 12 The following primary antibodies were used: rabbit polyclonal anti-CysC (Epitomics Inc), mouse monoclonal anti-mannose-binding lectin protein C (anti-MBLC; Abcam), and mouse monoclonal anti-β-tubulin (Huaan Biotech). Immunoreactive bands were visualized using enhanced chemiluminescence horseradish peroxidase substrate (Millipore Corporation). The optical density was determined using Image Lab 4.1 software.