The BALB/3T3 clone A31 cell line was purchased from American Type Culture Collection (Manassas, VA, USA) and maintained in Dulbecco’s Modified Eagle’s Medium containing 4.5 g/L glucose and 4 mM L-glutamine (Lonza, Walkedrsville, MD, USA) and supplemented with 10% fetal bovine serum (Lonza) at 37°C in a humidified atmosphere consisting of 5% CO2 in air. Microspheres were added at a final concentration of 0.33 mg/mL (unless otherwise described) to a monolayer of 3T3 cells grown for 24 hours in six-well culture plates seeded with 105 cells per well or in a 60 mm culture dish seeded with 106 cells. The cells were further incubated for a given amount of time and then washed twice with phosphate-buffered saline (PBS). Cells were detached by trypsin–ethylenediaminetetraacetic acid treatment for flow cytometric analysis.
Balb 3t3 clone a31 cell line
Manufactured by American Type Culture Collection
Sourced in United States, Germany
The BALB/3T3 clone A31 cell line is a well-established mouse embryo fibroblast cell line. It is a commonly used tool in cell biology research.
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Lab products found in correlation
L glutamine, by Lonza (1 mentions)
Fetal bovine serum (fbs), by Lonza (1 mentions)
Mucin from porcine stomach type 2, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem, by Merck Group (1 mentions)
Ethylenediaminetetraacetic acid edta, by Merck Group (1 mentions)
Thiourea, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Trypsin, by Merck Group (1 mentions)
L glutamine, by Merck Group (1 mentions)
Acetone, by Merck Group (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Dmso d6, by Deutero (1 mentions)
Deuterated water d2o, by Deutero (1 mentions)
2 protocols using balb 3t3 clone a31 cell line
1
BALB/3T3 Cell Culture and Microsphere Assay
2
Cyclodextrin-Enhanced Dexamethasone Formulation
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Deuterated water (D2O, 99.9%) and dimethyl sulfoxide (DMSO-d6, 99.9%) were purchased from Deutero GmbH (Kastellaun, Germany). Additionally, 2-Methyl- β-cyclodextrin (MβCD) was kindly provided by Roquette Italia (Alessandria, Italy; MW 1191 g/mol, degree of C2 substitution ~0.5, corresponding to ~4 methyl groups per cyclodextrin molecule). Dexamethasone (Dex), acetic acid, thiourea (≥99.0%), hydrochloric acid 37%, acetone (≥99.0%), Sephadex® G-15 resin and mucin from porcine stomach type II were purchased from Merck (Darmstadt, Germany).
The fibroblast BALB/3T3 clone A31cell line was obtained from American Type Culture Collection, Dulbecco’s Modified Eagle’s Medium (DMEM), supplemented with 2 mM L-glutamine and 1% penicillin/streptomycin and 10% calf bovine serum, trypsin and ethylenediaminetetraacetic acid (EDTA) were obtained from Merck (Darmstadt, Germany).
The fibroblast BALB/3T3 clone A31cell line was obtained from American Type Culture Collection, Dulbecco’s Modified Eagle’s Medium (DMEM), supplemented with 2 mM L-glutamine and 1% penicillin/streptomycin and 10% calf bovine serum, trypsin and ethylenediaminetetraacetic acid (EDTA) were obtained from Merck (Darmstadt, Germany).
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