TC cell lines BHT-101, IHH4, TPC-1, CGTHW-3 and human normal thyroid cell Nthy-ori 3-1 were from American Type Culture Collection (ATCC; Manassas, VA, U.S.A.). Total RNA was extracted using TRIzol (Invitrogen, Carlsbad, CA, U.S.A.). The obtained total RNA was reverse transcribed into complementary DNA (cDNA) using reverse transcription kits (Thermo Scientific, Waltham, MA, U.S.A.): TaqMan™ MicroRNA Reverse Transcription Kit (4366596), High-Capacity cDNA Reverse Transcription Kit (4368813). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and U6 (Invitrogen, Carlsbad, CA, U.S.A.) were taken as internal references. The primers were designed and synthesized by Invitrogen company (Carlsbad, CA, U.S.A.) (Table 1 ). The final data were analyzed by the 2−ΔΔCt method. Cell lines with low expression of miR-143-3p and high expression of LINC01296 and MSI2 were screened out.
Cgthw 3
Manufactured by American Type Culture Collection
Sourced in United States
The CGTHW-3 is a laboratory equipment product offered by American Type Culture Collection. It is designed for the purpose of culturing and maintaining cell growth. The core function of the CGTHW-3 is to provide a controlled and optimized environment for the cultivation of various cell types.
Automatically generated - may contain errors
Lab products found in correlation
Tpc 1, by American Type Culture Collection (2 mentions)
Ihh 4, by American Type Culture Collection (2 mentions)
High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (2 mentions)
Reverse transcription kit, by Thermo Fisher Scientific (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Taqman microrna reverse transcription kit, by Thermo Fisher Scientific (1 mentions)
Nthy ori 3 1, by American Type Culture Collection (1 mentions)
Bht101, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
2 protocols using cgthw 3
1
Quantitative Analysis of miR-143-3p, LINC01296, and MSI2 in Thyroid Cancer
2
Papillary Thyroid Carcinoma Tissue Collection
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TPC sample. Twenty-five pairs of human PTC and adjacent normal tissues were obtained from the Second Affiliated Hospital of Xi'an Jiaotong University (Shaanxi, China). The tissues were frozen in liquid nitrogen and stored at -80˚C until use. written informed consent for tissue donation (for research purposes) was obtained from the patients, and the protocol was approved by the Institutional Review board of the Second Affiliated Hospital of Xi'an Jiaotong University.
Cell lines. Four human PTC cell lines, including TPC-1, K1, IHH-4 and CGTH-w3, were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA). The human thyroid epithelial cell lines Nthy-ori3-1 and HTori-3 were purchased from Shanghai Cell Collection (SCC; Shanghai, China). These cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM) plus 10% fetal bovine serum (FBS; Life Technologies, Inc., Grand Island, NY, USA) at 37˚C in a humidified atmosphere containing 5% CO 2 .
Cell lines. Four human PTC cell lines, including TPC-1, K1, IHH-4 and CGTH-w3, were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA). The human thyroid epithelial cell lines Nthy-ori3-1 and HTori-3 were purchased from Shanghai Cell Collection (SCC; Shanghai, China). These cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM) plus 10% fetal bovine serum (FBS; Life Technologies, Inc., Grand Island, NY, USA) at 37˚C in a humidified atmosphere containing 5% CO 2 .
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