The HaCaT human skin keratinocyte cell line was purchased from the American Type Culture Collection (Manassas, VA, USA). The cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Invitrogen, Carlsbad, CA, USA) supplemented with 10% FBS, 100 U/ml penicillin, and 0.1 mg/ml streptomycin at 37 °C in a humidified incubator with 5% CO2.
Hacat human skin keratinocyte cell line
Manufactured by American Type Culture Collection
Sourced in United States
The HaCaT human skin keratinocyte cell line is a well-characterized and widely used in vitro model of human epidermal keratinocytes. It is a spontaneously immortalized, nontumorigenic human keratinocyte cell line that retains many characteristics of normal human keratinocytes.
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2 protocols using hacat human skin keratinocyte cell line
1
HaCaT Keratinocyte Cell Maintenance
2
Culturing HaCaT Human Skin Keratinocytes
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HaCaT human skin keratinocyte cell line was obtained from the American Type Culture Collection (Manassas, MD, USA) and cultured in RPMI 1640 medium (WelGENE Inc., Daegu, Republic of Korea) containing 10 % heat-inactivated fetal bovine serum (FBS, WelGENE Inc., Daegu, Republic of Korea), streptomycin (100 μg/mL), and penicillin (100 Units/mL). Cells were maintained at 37 °C in an incubator with a humidified atmosphere of 5 % CO2.
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