Detailed methods are provided in the online version of this paper and include the following: ). Core body temperature was maintained at 37 ± 0.5 C throughout the procedure with a homeothermic system (Harvard Apparatus, UK). A vertical incision between the left eye and ear was made and the main trunk and bifurcations of the middle cerebral artery exposed via a subtemporal craniectomy. The site was cooled continuously with saline application. The middle cerebral artery was electrocoagulated at the junction of its main trunk and bifurcation and cessation of blood flow confirmed visually prior to cutting through the coagulated area. The temporal muscle was repositioned, the incision sutured and topical local anesthetic (lidocaine/prilocaine, LMX4) applied. Anesthesia was discontinued and mice were administered 0.5 mL sterile saline subcutaneously. Mice were returned to home cages on a heating blanket and then transferred to pre-surgery holding areas.
Homeothermic system
Manufactured by Harvard Apparatus
Sourced in United Kingdom, United States
The Homeothermic system is a device designed to maintain a constant temperature environment for various experimental applications. It regulates the temperature of the sample or specimen under observation to ensure a stable and controlled thermal environment.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using homeothermic system
1
Middle Cerebral Artery Occlusion in Mice
2
Rat Middle Cerebral Artery Occlusion
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A stroke was induced using the filament model as described previously
19 (link)–
21 (link)
. Briefly, rats were anaesthetised using isoflurane in a 70:30 nitrous oxide: oxygen mixture. Body temperature was maintained at 37°C using a homeothermic system (Harvard Apparatus, US). A silicone-coated nylon filament (diameter: 0.39–0.41mm, Doccol, USA) was advanced up the right common carotid, into the internal carotid artery, until a decrease in blood flow was observed with a Laser Doppler probe (Moor Instruments, UK) against the temporal bone. Rats were recovered, functional deficits noted, and briefly re-anaesthetised after 30 minutes to remove the filament and ligate the common carotid. Local anaesthetic was applied to the surgical site and rats were given 1mg/ml paracetamol in the drinking water from 12h prior to until 48h after surgery along with mashed diet. Subcutaneous injections of saline (5mg/kg) and atropine (0.1mg/kg, Animalcare Ltd., UK) were given at the time of surgery. This procedure was completed during daylight hours from 8am until 5pm.
19 (link)–
21 (link)
. Briefly, rats were anaesthetised using isoflurane in a 70:30 nitrous oxide: oxygen mixture. Body temperature was maintained at 37°C using a homeothermic system (Harvard Apparatus, US). A silicone-coated nylon filament (diameter: 0.39–0.41mm, Doccol, USA) was advanced up the right common carotid, into the internal carotid artery, until a decrease in blood flow was observed with a Laser Doppler probe (Moor Instruments, UK) against the temporal bone. Rats were recovered, functional deficits noted, and briefly re-anaesthetised after 30 minutes to remove the filament and ligate the common carotid. Local anaesthetic was applied to the surgical site and rats were given 1mg/ml paracetamol in the drinking water from 12h prior to until 48h after surgery along with mashed diet. Subcutaneous injections of saline (5mg/kg) and atropine (0.1mg/kg, Animalcare Ltd., UK) were given at the time of surgery. This procedure was completed during daylight hours from 8am until 5pm.
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