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2 protocols using s aureus mtcc 737

1

Antimicrobial Activity of Soil Isolate

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The wild-type soil-isolate (URID 12.1) showing antimicrobial activity was grown in modified Tryptone Soya Broth (mTSB, with 0.5% yeast extract) at 37°C under shaking conditions (110 rpm). All indicator bacterial strains (Tables 1, 4) including the quality control strains S. aureus ATCC 29213 and S. aureus MTCC 737 (ATCC 6538P) and E. faecalis ATCC 29212, were subcultured in Mueller-Hinton and Brain Heart Infusion (BHI) broths at 37°C. The quality control strains S. aureus ATCC 29213 and E. faecalis ATCC 29212 were obtained from OncQuest Laboratory, Indore, whereas the S. aureus MTCC 737 (ATCC 6538P) was procured from the Microbial Type Culture Collection (MTCC), Chandigarh, India. All the cultures used as indicator strains (Tables 1, 4) were obtained from the MTCC, Chandigarh and National Collection of Industrial Microorganisms (NCIM), Pune, and the clinical isolates from the Central Laboratory, OncQuest Laboratory, Indore, and S. aureus GMC from Goa Medical College, India. The cultures were also maintained as 20% glycerol stocks at −80°C. The antimicrobial susceptibilities of the quality control strains were tested using oxacillin and vancomycin.
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2

Antimicrobial Potential of Marine Bacteria

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Antimicrobial potential of marine fish bacterial isolates was evaluated against common fish pathogens such as Aeromonas hydrophila, Vibrio alginolyticus, Vibrio parahaemolyticus and Staphylococcus aureus were obtained from Central Marine Fisheries Research Institute (CMFRI), Mandapam, Tamil Nadu, India, and other strains, Shigella flexneri MTCC 1457, Enterococcus. faecalis MTCC 439, A. hydrophila MTCC 1739, Escherichia coli MTCC 40, Pseudomonas aeruginosa MTCC 4679, S. aureus MTCC 737, Klebsiella pneumoniae MTCC 39 and Proteus vulgaris MTCC 426 were purchased from Microbial Type Culture Collection (MTCC), Chandigarh, India.
The Bacillus subtilis culture was purified from the commercially available fish probiotic product and confirmed by morphological and biochemical characterization. A single colony of purified B. subtilis was cultured and used as positive control.
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