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Anti ucp1 polyclonal antibody

Manufactured by Santa Cruz Biotechnology
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Sourced in United States

The Anti-UCP1 polyclonal antibody is a laboratory reagent designed for the detection and analysis of the Uncoupling Protein 1 (UCP1) in biological samples. UCP1 is a mitochondrial protein involved in thermogenesis and energy expenditure. This antibody can be used in various applications, such as Western blotting, immunohistochemistry, and immunoprecipitation, to study the expression and localization of UCP1 in cells and tissues.

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Lab products found in correlation

Irdye 800cw goat anti rabbit, by LI COR (2 mentions) Anti prdm16 polyclonal antibody abd130, by Merck Group (2 mentions) Irdye 680rd goat anti rabbit, by LI COR (2 mentions) Alexa fluor 488, by Cell Signaling Technology (2 mentions) β tubulin, by Cell Signaling Technology (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) 3 isobutyl 1 methylxanthine, by Merck Group (2 mentions) Oil red o, by Merck Group (2 mentions) Dmem f12, by Thermo Fisher Scientific (2 mentions) Compound c, by Merck Group (2 mentions) Insulin, by Merck Group (2 mentions) Dexamethasone (dex), by Merck Group (2 mentions) Indomethacin, by Merck Group (2 mentions) Dispase 2, by Roche (2 mentions) Collagenase d, by Roche (2 mentions) Image pro plus 6, by Media Cybernetics (1 mentions)

Close spelling variants of this product

Anti-UCP1 antibody Anti-UCP1

3 protocols using anti ucp1 polyclonal antibody

1

Adipogenic Differentiation Antibody Reagents

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Antibodies against AMPKα (#2532), phospho-AMPKα at Thr172 (#2535), pyruvate dehydrogenase (PDH) (#2784), cytochrome c (#4280), β-tubulin (#2146) and goat anti-rat antibody Alexa Fluor 488 (#4416) were purchased from Cell Signaling (Danvers, MA). Anti-PRDM16 polyclonal antibody (#ABD130) was purchased from Millipore (Billerica, MA). Anti-UCP1 polyclonal antibody (#sc28766) was bought from Santa Cruz Biotechnology (Dallas, TX). Goat anti-rabbit IRDye 800CW (#926-32211) and goat anti-rabbit IRDye 680RD (#926-68070) secondary antibodies for western blot were purchased from LI-COR (Lincoln, NE). Fluoro-Gel II with 40, 6-diamidino-2-phenylindole (DAPI) (#17985-50) was purchased from Electron Microscopy Sciences (Hatfield, PA). Insulin, dexamethasone, indomethacin, 3-isobutyl-1-methylxanthine (IBMX), Triiodothyronine (T3), Oil-Red O, and compound C were purchased from Sigma (St. Louis, MO). Collagenase D and dispase II were purchased from Roche Diagnostics (Indianapolis, IN). DMEM/F12 and fetal bovine serum (FBS) were purchased from Life Technologies (Grand Island, NY).
Wang S., Liang X., Yang Q., Fu X., Rogers C.J., Zhu M., Rodgers B.D., Jiang Q., Dodson M.V, & Du M. (2015). Resveratrol induces brown-like adipocyte formation in white fat through activation of AMP-activated protein kinase (AMPK) α1. International journal of obesity (2005), 39(6), 967-976.
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2

Histological Analysis of iBAT

Cited 1 time
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H&E staining of iBAT and immunostaining of the differentiated iBAT SVCs and iBAT sections were conducted as previously described [29 (link)]. The anti-UCP1 polyclonal antibody was purchased from Santa Cruz Biotechnology (Dallas, TX, USA) and used at a dilution of 1:200. Adipocyte diameters were measured by Image-Pro Plus 6.0 (Media Cybernetics, Inc., Rockville, MD).
Wang S., Liang X., Yang Q., Fu X., Zhu M., Rodgers B.D., Jiang Q., Dodson M.V, & Du M. (2017). Resveratrol enhances brown adipocyte formation and function by activating AMP-activated protein kinase (AMPK) α1 in mice fed high-fat diet. Molecular nutrition & food research, 61(4), 10.1002/mnfr.201600746.
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3

Adipogenic Differentiation Antibody Reagents

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies against AMPKα (#2532), phospho-AMPKα at Thr172 (#2535), pyruvate dehydrogenase (PDH) (#2784), cytochrome c (#4280), β-tubulin (#2146) and goat anti-rat antibody Alexa Fluor 488 (#4416) were purchased from Cell Signaling (Danvers, MA). Anti-PRDM16 polyclonal antibody (#ABD130) was purchased from Millipore (Billerica, MA). Anti-UCP1 polyclonal antibody (#sc28766) was bought from Santa Cruz Biotechnology (Dallas, TX). Goat anti-rabbit IRDye 800CW (#926-32211) and goat anti-rabbit IRDye 680RD (#926-68070) secondary antibodies for western blot were purchased from LI-COR (Lincoln, NE). Fluoro-Gel II with 40, 6-diamidino-2-phenylindole (DAPI) (#17985-50) was purchased from Electron Microscopy Sciences (Hatfield, PA). Insulin, dexamethasone, indomethacin, 3-isobutyl-1-methylxanthine (IBMX), Triiodothyronine (T3), Oil-Red O, and compound C were purchased from Sigma (St. Louis, MO). Collagenase D and dispase II were purchased from Roche Diagnostics (Indianapolis, IN). DMEM/F12 and fetal bovine serum (FBS) were purchased from Life Technologies (Grand Island, NY).
Wang S., Liang X., Yang Q., Fu X., Rogers C.J., Zhu M., Rodgers B.D., Jiang Q., Dodson M.V, & Du M. (2015). Resveratrol induces brown-like adipocyte formation in white fat through activation of AMP-activated protein kinase (AMPK) α1. International journal of obesity (2005), 39(6), 967-976.
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