To acquire the infrared (IR) spectra, we used a Shimadzu Fourier Transform Infrared spectrometer with KBr pellets. The ultraviolet (UV) spectra were acquired using a double beam spectrophotometer (UH5300). The optical rotations were acquired using a Rudolph Autopol IV polarimeter (Hackettstown, NJ, USA). High-resolution electrospray ionization mass spectra (HRESIMS) were obtained using an Agilent 6200 Q-TOF MS system or a Thermo Scientific Q Exactive Orbitrap MS system. Nuclear magnetic resonance (NMR) spectra were obtained using a Bruker Avance III 600 MHz spectrometer (Bruker, Karlsruhe, Germany). Column chromatography (CC) was performed using a Sephadex LH-20 (GE Healthcare) with silica gel (80–100 and 200–300 mesh) and RP-18 gel (20–45 μm; FuJi). High-performance liquid chromatography (HPLC) was performed using an Agilent 1260 liquid chromatography system equipped with Zorbax SB-C18 columns (5 μm; 9.4 mm × 150 mm or 21.2 mm × 150 mm).
6200 q tof ms system
Manufactured by Thermo Fisher Scientific
The 6200 Q-TOF MS system is a high-resolution, accurate-mass mass spectrometer designed for analytical applications. It features a quadrupole time-of-flight (Q-TOF) mass analyzer that provides accurate mass measurements and high resolving power. The system is capable of performing a range of mass spectrometry techniques, including full-scan, targeted, and data-dependent acquisition modes.
Automatically generated - may contain errors
Lab products found in correlation
Q exactive orbitrap ms system, by Thermo Fisher Scientific (3 mentions)
1260 liquid chromatography system, by Agilent Technologies (3 mentions)
Silica gel, by Qingdao Haiyang Chemical (3 mentions)
Gf254, by Qingdao Haiyang Chemical (3 mentions)
Fourier transform infrared spectrometer, by Shimadzu (2 mentions)
Avance 3 600 mhz spectrometer, by Bruker (2 mentions)
Sb c18 column, by Agilent Technologies (1 mentions)
Sepa 300 polarimeter, by Horiba (1 mentions)
Uh5300, by Hitachi (1 mentions)
3 protocols using 6200 q tof ms system
1
Spectroscopic Characterization of Organic Compounds
2
Spectroscopic Characterization of Compounds
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Optical rotations (ORs)
were measured using a Horiba SEPA-300 polarimeter. Infrared (IR) spectra
were obtained with a Tenor 27 spectrophotometer using KBr pellets.
One-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance
(NMR) spectra were recorded using a Bruker Avance III 600 MHz spectrometer
with tetramethylsilane as an internal standard. Chemical shifts (δ)
were expressed in ppm with reference to the solvent signals. High-resolution
electrospray ionization mass spectra (HRESIMS) were recorded on an
Agilent 6200 Q-TOF MS system or a Thermo Scientific Q Exactive Orbitrap
MS system. Column chromatography (CC) was performed on silica gel
(200–300 mesh, Qingdao Haiyang Chemical Co., Ltd., China),
reverse phase-C18 gel (20–45 μm, Fuji), and
Sephadex LH-20 (GE Healthcare, Sweden). Medium pressure liquid chromatography
(MPLC) was performed using Biotage SP1 equipment and columns packed
with RP-C18 gel. Preparative high-performance liquid chromatography
(prep-HPLC) was performed using an Agilent 1260 liquid chromatography
system equipped with Zorbax SB-C18 columns (5 μm, 9.4 mm ×
150 mm, or 21.2 mm × 150 mm) and a diode array detector (DAD).
Fractions were monitored using the DAD detector or thin layer chromatography
(TLC) (GF 254, Qingdao Haiyang Chemical Co., Ltd., China).
were measured using a Horiba SEPA-300 polarimeter. Infrared (IR) spectra
were obtained with a Tenor 27 spectrophotometer using KBr pellets.
One-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance
(NMR) spectra were recorded using a Bruker Avance III 600 MHz spectrometer
with tetramethylsilane as an internal standard. Chemical shifts (δ)
were expressed in ppm with reference to the solvent signals. High-resolution
electrospray ionization mass spectra (HRESIMS) were recorded on an
Agilent 6200 Q-TOF MS system or a Thermo Scientific Q Exactive Orbitrap
MS system. Column chromatography (CC) was performed on silica gel
(200–300 mesh, Qingdao Haiyang Chemical Co., Ltd., China),
reverse phase-C18 gel (20–45 μm, Fuji), and
Sephadex LH-20 (GE Healthcare, Sweden). Medium pressure liquid chromatography
(MPLC) was performed using Biotage SP1 equipment and columns packed
with RP-C18 gel. Preparative high-performance liquid chromatography
(prep-HPLC) was performed using an Agilent 1260 liquid chromatography
system equipped with Zorbax SB-C18 columns (5 μm, 9.4 mm ×
150 mm, or 21.2 mm × 150 mm) and a diode array detector (DAD).
Fractions were monitored using the DAD detector or thin layer chromatography
(TLC) (GF 254, Qingdao Haiyang Chemical Co., Ltd., China).
3
Characterization of Natural Products
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IR spectra were obtained on a Shimadzu Fourier transform infrared spectrometer using KBr pellets. UV spectra were obtained by using a double beam spectrophotometer UH5300 (Hitachi High-Technologies, Tokyo, Japan). Optical rotations were measured on a Rudolph Autopol IV polarimeter (Hackettstown, NJ, USA). High resolution electrospray ionization mass spectra (HRESIMS) were recorded on an Agilent 6200 Q-TOF MS system or a Thermo Scientific Q Exactive Orbitrap MS system. Circular dichroism (CD) spectra were measured with an Applied Photophysics spectrometer (Chirascan, New Haven, CT, USA). NMR spectra were recorded with a Bruker Avance III 600 MHz spectrometer (Bruker, Karlsruhe, Germany). Sephadex LH-20 (GE Healthcare, Pittsburgh, PA, USA), Silica gel (200–300 mesh), and RP-18 gel (20–45 μM, FuJi) were used for column chromatography (CC). HPLC was performed on an Agilent 1260 liquid chromatography system equipped with Zorbax SB-C18 columns (5 μM, 9.4 mm × 150 mm, or 21.2 mm × 150 mm).
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